TY - JOUR
T1 - A reusable copper-cysteamine fluorescence probe for cost-effective detection of alkaline phosphatase activity based on a redox-modulated inner filter effect
AU - Li, Yue
AU - Cai, Yanting
AU - Wang, Yuanyuan
AU - Xue, Ruisong
AU - Ren, Ziwei
AU - Liu, Yanmei
AU - Chen, Wei
AU - Liu, Zhen
AU - Bao, Xingfu
AU - Huang, Zhenzhen
N1 - Publisher Copyright:
© 2025 Elsevier B.V.
PY - 2025/2
Y1 - 2025/2
N2 - The cost-effective and eco-friendly monitoring of alkaline phosphatase (ALP) activity is crucial for the diagnosis and treatment of various diseases. In this study, we investigated the utilization of a two-dimensional (2D) sheet-like copper-cysteamine (Cu-Cy) as a fluorescence probe for the detection of ALP activity. It was found that the fluorescence emission of Cu-Cy could be reversibly switched off and on by the alternate addition of manganese dioxide nanosheets (MnO2 NSs) and ascorbic acid (AA) based on a redox-modulated inner filter effect. Additionally, owing to its 2D structure and exceptional stability, Cu-Cy exhibited effective reusability for at least four cycles without any noticeable loss in sensing performance. Based on these findings, an efficient fluorescent approach was designed for ALP detection by monitoring the generation of AA through the hydrolysis of L-ascorbic acid 2-phosphate catalyzed by ALP. The assay achieved a detection limit of 0.1 U/L with a linear detection range from 1 to 100 U/L. Furthermore, this sensing method enabled the detection of ALP in human serum and saliva samples, as well as distinguishing between the saliva of healthy individuals and patients with periodontitis. Moreover, this approach demonstrated significant potential for ALP inhibitor screening, thus offering promising prospects for clinical diagnosis and treatment of ALP-related diseases.
AB - The cost-effective and eco-friendly monitoring of alkaline phosphatase (ALP) activity is crucial for the diagnosis and treatment of various diseases. In this study, we investigated the utilization of a two-dimensional (2D) sheet-like copper-cysteamine (Cu-Cy) as a fluorescence probe for the detection of ALP activity. It was found that the fluorescence emission of Cu-Cy could be reversibly switched off and on by the alternate addition of manganese dioxide nanosheets (MnO2 NSs) and ascorbic acid (AA) based on a redox-modulated inner filter effect. Additionally, owing to its 2D structure and exceptional stability, Cu-Cy exhibited effective reusability for at least four cycles without any noticeable loss in sensing performance. Based on these findings, an efficient fluorescent approach was designed for ALP detection by monitoring the generation of AA through the hydrolysis of L-ascorbic acid 2-phosphate catalyzed by ALP. The assay achieved a detection limit of 0.1 U/L with a linear detection range from 1 to 100 U/L. Furthermore, this sensing method enabled the detection of ALP in human serum and saliva samples, as well as distinguishing between the saliva of healthy individuals and patients with periodontitis. Moreover, this approach demonstrated significant potential for ALP inhibitor screening, thus offering promising prospects for clinical diagnosis and treatment of ALP-related diseases.
KW - Alkaline phosphatase
KW - Copper-cysteamine
KW - Fluorescence detection
KW - MnO nanosheets
KW - Reusable fluorescence probe
UR - http://www.scopus.com/inward/record.url?scp=85214338222&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2025.112694
DO - 10.1016/j.microc.2025.112694
M3 - Article
AN - SCOPUS:85214338222
SN - 0026-265X
VL - 209
JO - Microchemical Journal
JF - Microchemical Journal
M1 - 112694
ER -
