TY - JOUR
T1 - A putative association of interleukin-1β promoter polymorphisms and il-1β levels in Saudi diabetic patients
AU - Tabrez, Shams
AU - Md. Ashraf, Ghulam
AU - Hindawi, Salwa
N1 - Publisher Copyright:
© 2018 Begell House, Inc.
PY - 2018
Y1 - 2018
N2 - Type 2 diabetes mellitus (T2DM) is a well-known endocrine disorder affecting a significant number of individuals across the globe. Risk factors such as inflammation, obesity, high blood glucose level, cardiovascular disease, and genetic alteration are believed to be the reason for T2DM onset. The current study was intended to envisage the possible association between polymorphisms in the interleukin-1β (IL-1β) promoter and IL-1β serum levels in Saudi T2DM patients. Biochemical parameters such as fasting blood glucose (FBS), percentage glycosylated hemoglobin (HbA1c), and lipid profile were measured spectrophotometrically. Serum insulin levels were measured using an immunochemistry analyzer. The commercial enzyme-linked immune assay (ELISA) kit was used to estimate the level of IL-1β in the serum samples. The Sanger sequencing method was adopted to determine single-nucleotide polymorphisms (SNPs) in the IL-1β promoter. We observed a significant (P < 0.001) elevation in FBS, Hb1Ac, insulin, and low-density lipoprotein cholesterol in T2DM patients compared with control individuals. Similarly, IL-1β level were found to be increased by 221% in T2DM patients compared with controls. A nonsignificant genotypic association was observed in the IL-1β gene at the −511C/T and −31C/TProopositions in T2DM patients compared with control individuals. However, a significant association (P < 0.05) was observed at the allelic level. Further studies on larger sample sizes are recommended to establish the exact role of IL-1β polymorphism in the pathogenesis of T2DM and its genetic risk.
AB - Type 2 diabetes mellitus (T2DM) is a well-known endocrine disorder affecting a significant number of individuals across the globe. Risk factors such as inflammation, obesity, high blood glucose level, cardiovascular disease, and genetic alteration are believed to be the reason for T2DM onset. The current study was intended to envisage the possible association between polymorphisms in the interleukin-1β (IL-1β) promoter and IL-1β serum levels in Saudi T2DM patients. Biochemical parameters such as fasting blood glucose (FBS), percentage glycosylated hemoglobin (HbA1c), and lipid profile were measured spectrophotometrically. Serum insulin levels were measured using an immunochemistry analyzer. The commercial enzyme-linked immune assay (ELISA) kit was used to estimate the level of IL-1β in the serum samples. The Sanger sequencing method was adopted to determine single-nucleotide polymorphisms (SNPs) in the IL-1β promoter. We observed a significant (P < 0.001) elevation in FBS, Hb1Ac, insulin, and low-density lipoprotein cholesterol in T2DM patients compared with control individuals. Similarly, IL-1β level were found to be increased by 221% in T2DM patients compared with controls. A nonsignificant genotypic association was observed in the IL-1β gene at the −511C/T and −31C/TProopositions in T2DM patients compared with control individuals. However, a significant association (P < 0.05) was observed at the allelic level. Further studies on larger sample sizes are recommended to establish the exact role of IL-1β polymorphism in the pathogenesis of T2DM and its genetic risk.
KW - Cytokines
KW - Diabetes
KW - Inflammation
KW - Interleukins
KW - Polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85054775852&partnerID=8YFLogxK
U2 - 10.1615/CritRevEukaryotGeneExpr.2018025757
DO - 10.1615/CritRevEukaryotGeneExpr.2018025757
M3 - Article
C2 - 30311584
AN - SCOPUS:85054775852
SN - 1045-4403
VL - 28
SP - 349
EP - 356
JO - Critical Reviews in Eukaryotic Gene Expression
JF - Critical Reviews in Eukaryotic Gene Expression
IS - 4
ER -
