TY - JOUR
T1 - A novel 3D method of locomotor analysis in adult zebrafish
T2 - Implications for automated detection of CNS drug-evoked phenotypes
AU - Stewart, Adam Michael
AU - Grieco, Fabrizio
AU - Tegelenbosch, Ruud A.J.
AU - Kyzar, Evan J.
AU - Nguyen, Michael
AU - Kaluyeva, Aleksandra
AU - Song, Cai
AU - Noldus, Lucas P.J.J.
AU - Kalueff, Allan V.
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/11/30
Y1 - 2015/11/30
N2 - Background: Expanding the spectrum of organisms to model human brain phenotypes is critical for our improved understanding of the pathobiology of neuropsychiatric disorders. Given the clear limitations of existing mammalian models, there is an urgent need for low-cost, high-throughput in-vivo technologies for drug and gene discovery. New method: Here, we introduce a new automated method for generating 3D (X,Y,Z) swim trajectories in adult zebrafish (Danio rerio), to improve their neurophenotyping. Results: Based on the Track3D module of EthoVision XT video tracking software (Noldus Information Technology), this tool enhances the efficient, high-throughput 3D analyses of zebrafish behavioral responses. Applied to adult zebrafish behavior, this 3D method is highly sensitive to various classes of psychotropic drugs, including selected psychostimulant and hallucinogenic agents. Comparison with existing Methods: Our present method offers a marked advance in the existing 2D and 3D methods of zebrafish behavioral phenotyping, minimizing research time and recording high-resolution, automatically synchronized videos with calculated, high-precision object positioning. Conclusions: Our novel approach brings practical simplicity and 'integrative' capacity to the often complex and error-prone quantification of zebrafish behavioral phenotypes. Illustrating the value of 3D swim path reconstructions for identifying experimentally-evoked phenotypic profiles, this method fosters innovative, ethologically relevant, and fully automated small molecule screens using adult zebrafish.
AB - Background: Expanding the spectrum of organisms to model human brain phenotypes is critical for our improved understanding of the pathobiology of neuropsychiatric disorders. Given the clear limitations of existing mammalian models, there is an urgent need for low-cost, high-throughput in-vivo technologies for drug and gene discovery. New method: Here, we introduce a new automated method for generating 3D (X,Y,Z) swim trajectories in adult zebrafish (Danio rerio), to improve their neurophenotyping. Results: Based on the Track3D module of EthoVision XT video tracking software (Noldus Information Technology), this tool enhances the efficient, high-throughput 3D analyses of zebrafish behavioral responses. Applied to adult zebrafish behavior, this 3D method is highly sensitive to various classes of psychotropic drugs, including selected psychostimulant and hallucinogenic agents. Comparison with existing Methods: Our present method offers a marked advance in the existing 2D and 3D methods of zebrafish behavioral phenotyping, minimizing research time and recording high-resolution, automatically synchronized videos with calculated, high-precision object positioning. Conclusions: Our novel approach brings practical simplicity and 'integrative' capacity to the often complex and error-prone quantification of zebrafish behavioral phenotypes. Illustrating the value of 3D swim path reconstructions for identifying experimentally-evoked phenotypic profiles, this method fosters innovative, ethologically relevant, and fully automated small molecule screens using adult zebrafish.
KW - Drug discovery
KW - High-throughput screening
KW - Neurophenotype
KW - Video tracking
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=84941768968&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2015.07.023
DO - 10.1016/j.jneumeth.2015.07.023
M3 - Article
C2 - 26238728
AN - SCOPUS:84941768968
SN - 0165-0270
VL - 255
SP - 66
EP - 74
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
ER -