A Meta-analysis: Evaluating the Effect of METTL3/METTL14 on m6A Level Based on Knockdown Samples

Yuxin Zhang, Yuxuan Wu, Di Zhen, Kunqi Chen*, Jia Meng

*Corresponding author for this work

Research output: Chapter in Book or Report/Conference proceedingConference Proceedingpeer-review

Abstract

N6-methyladenosine (m6A) is a dynamic modification regulated by the m6A enzymes prevalent on mRNA. The Mettl3 and mettl14 are two subunits of methyltransferase. Different studies have shown that knockout of the METL3 and MTEL14 genes has the potential to change methylation levels. We have therefore undertaken a meta-analysis study to assess the quality and quantity of available evidence. The focus of the research would on the effects of mRNA methylation level after the depletion of METTL3 or METTL14. R package "Metafor"was applied to output the model of random effect, the regarding risk ratios (RRs), and the 95% confidence intervals (CIs). Owing to the high heterogeneity of the overall data (I2>99%), some samples were selected and excluded to an acceptable level. Three published trials for METTL3 (Q = 12.85, I2 = 37.8%) and two for METTL4 (Q = 45.48, I2 = 82.9%) were qualified to review and analyze. Consequently, we successfully proved the correlation between the knockdown of METTL3/METTL14 and modification of methylation level.

Original languageEnglish
Title of host publicationProceedings of the 5th International Conference on Biological Information and Biomedical Engineering, BIBE 2021
EditorsBin Chen
PublisherAssociation for Computing Machinery
ISBN (Electronic)9781450389297
DOIs
Publication statusPublished - 20 Jul 2021
Event5th International Conference on Biological Information and Biomedical Engineering, BIBE 2021 - Hangzhou, China
Duration: 20 Jul 202121 Jul 2021

Publication series

NameACM International Conference Proceeding Series

Conference

Conference5th International Conference on Biological Information and Biomedical Engineering, BIBE 2021
Country/TerritoryChina
CityHangzhou
Period20/07/2121/07/21

Keywords

  • Meta-analysis
  • Methylation level
  • Writers
  • m6A

Fingerprint

Dive into the research topics of 'A Meta-analysis: Evaluating the Effect of METTL3/METTL14 on m6A Level Based on Knockdown Samples'. Together they form a unique fingerprint.

Cite this