A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties

Ruipu Xin; Suijian Qi; Chaoxi Zeng; Faez Iqbal Khan; Bo Yang; Yonghua Wang

doi:10.1016/j.foodchem.2016.09.012

A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties

Ruipu Xin, Suijian Qi, Chaoxi Zeng, Faez Iqbal Khan, Bo Yang, Yonghua Wang^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

107 Citations (Scopus)

Abstract

In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and ¹H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.

Original language	English
Pages (from-to)	560-567
Number of pages	8
Journal	Food Chemistry
Volume	217
DOIs	https://doi.org/10.1016/j.foodchem.2016.09.012
Publication status	Published - 15 Feb 2017
Externally published	Yes

Keywords

Choline chloride
Deep eutectic solvents
Lysozyme
Physicochemical properties
Thermostability
Trehalose

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10.1016/j.foodchem.2016.09.012

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title = "A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties",

abstract = "In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.",

keywords = "Choline chloride, Deep eutectic solvents, Lysozyme, Physicochemical properties, Thermostability, Trehalose",

author = "Ruipu Xin and Suijian Qi and Chaoxi Zeng and Khan, {Faez Iqbal} and Bo Yang and Yonghua Wang",

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year = "2017",

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doi = "10.1016/j.foodchem.2016.09.012",

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T2 - Structural and physicochemical properties

AU - Xin, Ruipu

AU - Qi, Suijian

AU - Zeng, Chaoxi

AU - Khan, Faez Iqbal

AU - Yang, Bo

AU - Wang, Yonghua

PY - 2017/2/15

Y1 - 2017/2/15

N2 - In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.

AB - In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.

KW - Choline chloride

KW - Deep eutectic solvents

KW - Lysozyme

KW - Physicochemical properties

KW - Thermostability

KW - Trehalose

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DO - 10.1016/j.foodchem.2016.09.012

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SN - 0308-8146

VL - 217

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JO - Food Chemistry

JF - Food Chemistry

ER -

A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties

Abstract

Keywords

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