Abstract
This study aimed to evaluate the therapeutic properties of the traditional Chinese medicine Xuesanqi (XSQ,
from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many
active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various
signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice.
Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues.
The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content
was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot,
the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by westernblot.
Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss,
blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis
mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted
expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed
a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ
is a promising new treatment platform for ulcerative colitis.
from the rhizome of Polygonum amplexicaule D. Don) in treating ulcerative colitis. We hypothesized that its many
active components can alleviate symptoms of colitis by regulating the gut microbiota, its metabolites, and various
signaling pathways. To test our hypotheses, we designed a DSS- induced colitis model in C57BL/6 male mice.
Apparent metrics were evaluated in each group of mice and performed histological analysis of relevant tissues.
The gut microbial composition was analyzed by 16S rRNA sequencing of bacteria. Simultaneously, the SCFAs content
was detected by gas chromatography, inflammatory factor secretion was evaluated by ELISA or western-blot,
the expression of tight junction protein and key proteins of the MAPK signaling pathway were analyzed by westernblot.
Our result showed that the treatment with XSQ alleviated significant various symptoms such as weight loss,
blood in stool, and shortening of colon. In addition, XSQ treatment restored the dysregulated gut microbiota in colitis
mice, increased short chain fatty acids (SCFAs) and normalized the MAPK/ERK/JNK signaling pathways, promoted
expression of tight junction protein Occludin, Claudin-1, and E-cadherin proteins. Furthermore, we also observed
a dose-dependent pattern in these treatment responses. These findings demonstrated the active components of XSQ
is a promising new treatment platform for ulcerative colitis.
| Original language | English |
|---|---|
| Journal | Natural Products and Bioprospecting |
| Publication status | Published - Nov 2024 |