Video-aided analysis of zebrafish locomotion and anxiety-related behavioral responses

Jonathan M. Cachat, Peter R. Canavello, Salem I. Elkhayat, Brett K. Bartels, Peter C. Hart, Marco F. Elegante, Esther C. Beeson, Autumn L. Laffoon, Whitlee A.M. Haymore, David H. Tien, Anna K. Tien, Sopan Mohnot, Allan V. Kalueff

Research output: Chapter in Book or Report/Conference proceedingChapterpeer-review

58 Citations (Scopus)

Abstract

Due to physiological and anatomical similarities to other vertebrates, zebrafish are becoming a widely used model in neurobehavioral research. With the growing popularity of zebrafish as experimental subjects, it is important to develop tools that accurately record their behavioral phenotypes. Video-aided analysis of zebrafish behavior offers an increased spectrum of behavioral endpoints, some of which are not available using traditional (manual) observation. In addition, the use of computer software allows quantification of certain zebrafish behaviors that are otherwise highly subjective. This protocol describes a method for fast, accurate, and consistent video-aided measurements of zebrafish locomotion and anxiety-related behavior.

Original languageEnglish
Title of host publicationZebrafish Neurobehavioral Protocols
EditorsAllan Kalueff, Jonathan Cachat
Pages1-14
Number of pages14
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameNeuromethods
Volume51
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Behavioral endpoint
  • human error
  • manual observation
  • novel tank
  • video recording
  • video-tracking software

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