The role of mirnas in cisplatin-resistant hela cells

Yubo Yang, Cuihong Dai, Zhipeng Cai, Aiju Hou, Dayou Cheng, Dechang Xu*

*Corresponding author for this work

Research output: Chapter in Book or Report/Conference proceedingConference Proceedingpeer-review

1 Citation (Scopus)


Chemotherapy is the main strategy in the treatment of cancer, however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 and HeLa/CR4). According to the previous studies and exiting evidence, we selected five miRNAs (miR-183, miR-182, miR-30a, miR-15b and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was used to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines while miR-30a was significantly down-regulated. At the same time, the targets they regulated are related to the drug-resistance. The expression alteration of selected miRNAs in resistant cell lines compared to their parent HeLa cell line suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy.

Original languageEnglish
Title of host publicationBioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings
EditorsIon Măndoiu, Yaohang Li, Robert Harrison
PublisherSpringer Verlag
Number of pages11
ISBN (Print)9783319190471
Publication statusPublished - 2015
Externally publishedYes
Event11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015 - Norfolk, United States
Duration: 7 Jun 201510 Jun 2015

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349


Conference11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015
Country/TerritoryUnited States


  • Chemotherapy
  • Cisplatin-resistance
  • HeLa cell
  • MiRNAs


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