TY - JOUR
T1 - Role of PpIX-based photodynamic therapy in promoting the damage and apoptosis of colorectal cancer cell and its mechanisms
AU - Ouyang, Guoqing
AU - Liu, Zhipeng
AU - Xiong, Li
AU - Chen, Xiang
AU - Li, Qinglong
AU - Huang, He
AU - Lin, Liangwu
AU - Miao, Xiongying
AU - Ma, Lun
AU - Chen, Wei
AU - Wen, Yu
PY - 2017/8
Y1 - 2017/8
N2 - Objective: To explore the effects of protoporphyrin IX (PpIX)-mediated photodynamic therapy (PDT) on induction of apoptosis and death in colon cancer cell and the underlying mechanisms. Methods: The cell killing effect of PDT on HCT116 cell was determined by cell counting kit (CCK). The cells were divided into a control group, a single light group, a single PpIX group, and a PDT group. Hoechst 33342 and flow cytometry was used to assess the cell apoptosis. Western blot was employed to analyze the expressions of bcl-2, bax, and caspase-3. Reactive oxygen species (ROS) was detected by flow cytometry. Results: The viability of HCT116 cell was decreased gradually with the increase of irradiation dose (P<0.05). Compared to the other 3 groups, ROS production, the number of apoptotic cells and the protein expressions of bax and caspase-3 in PDT group increased, while bcl-2 expression was decreased (P<0.05). Conclusion: PpIX-mediated PDT can enhance the apoptosis in HCT116 cell, which may be related to mitochondrial apoptosis pathway.
AB - Objective: To explore the effects of protoporphyrin IX (PpIX)-mediated photodynamic therapy (PDT) on induction of apoptosis and death in colon cancer cell and the underlying mechanisms. Methods: The cell killing effect of PDT on HCT116 cell was determined by cell counting kit (CCK). The cells were divided into a control group, a single light group, a single PpIX group, and a PDT group. Hoechst 33342 and flow cytometry was used to assess the cell apoptosis. Western blot was employed to analyze the expressions of bcl-2, bax, and caspase-3. Reactive oxygen species (ROS) was detected by flow cytometry. Results: The viability of HCT116 cell was decreased gradually with the increase of irradiation dose (P<0.05). Compared to the other 3 groups, ROS production, the number of apoptotic cells and the protein expressions of bax and caspase-3 in PDT group increased, while bcl-2 expression was decreased (P<0.05). Conclusion: PpIX-mediated PDT can enhance the apoptosis in HCT116 cell, which may be related to mitochondrial apoptosis pathway.
KW - Apoptosis
KW - Colon cancer
KW - Killing
KW - Photodynamic therapy
KW - Protoporphyrin IX
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85030675561&partnerID=8YFLogxK
U2 - 10.11817/j.issn.1672-7347.2017.08.002
DO - 10.11817/j.issn.1672-7347.2017.08.002
M3 - Article
C2 - 28872077
AN - SCOPUS:85030675561
SN - 1672-7347
VL - 42
SP - 874
EP - 881
JO - Journal of Central South University (Medical Sciences)
JF - Journal of Central South University (Medical Sciences)
IS - 8
ER -
