[Real-time measurement of noradrenaline release in central nervous system].

Wei Huang*, Hong Ping Huang, Y. Mu, Lei Zhang, M. Jin, Jing Lv, Jing Li Gu, Yun Xiu, B. Zhang, Ning Guo, Tao Liu, Lei Sun, Mei Ying Song, Claire Xi Zhang, Huai Zhen Ruan, Zhuan Zhou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion, the dynamics of catecholamine release is studied in single cell, brain slice or in vivo. Noradrenaline is an important neurotransmitter and modulator in the central nervous system (CNS) and the peripheral nervous system (PNS). In the present paper, we first compared three real-time methods used to measure noradrenaline secretion in single cells (membrane capacitance, amperometry and confocal fluorescence microscopy imaging). Compared to the electrophysiological method and fluorescence microscopy, the basic usage of the carbon fiber electrode (CFE) in neuroscience research was presented as an example. Then, we presented a primary description of ion channels, including voltage-gated Na(+), K(+) and Ca(2+) channels in locus coeruleus (LC) neurons in rat brain slices. Finally, we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons, indicating Ca(2+)-dependent quantal noradrenaline release following Ca(2+) influx through Ca(2+) channels.

Original languageEnglish
Pages (from-to)865-870
Number of pages6
JournalSheng li xue bao : [Acta physiologica Sinica]
Issue number6
Publication statusPublished - 25 Dec 2007
Externally publishedYes

Cite this