TY - JOUR
T1 - Preliminary study on protein isolation process of walnut dregs by single factor experiments and response surface method
AU - Xue, Yufei
AU - Sun, Qian
AU - Ma, Zheng Feei
AU - Wang, Lin
AU - Kong, Lingming
N1 - Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2021/4
Y1 - 2021/4
N2 - The production of protein by alkali-solution and acid-isolation method will lead to the increase of salt in the feed liquid and affect its quality. In order to simplify the production of protein by the alkali acid precipitation process and obtain protein with high content and quality, the cation exchange resin was used to separate the walnut dreg protein. The desalination rate, protein content and recovery rate were used as indicators. Single factor experiments and response surface method were used to optimize the time used to isolate the protein from the cation exchange resin, the speed and temperature of the water bath shaker. Our results showed that the optimal time was determined at 62 min; the optimal speed of the water bath shaker was 120 rpm, and the optimal temperature was 49 °C. Under these conditions, the optimal protein recovery was 95.22% and the optimal protein content was 78.12%.
AB - The production of protein by alkali-solution and acid-isolation method will lead to the increase of salt in the feed liquid and affect its quality. In order to simplify the production of protein by the alkali acid precipitation process and obtain protein with high content and quality, the cation exchange resin was used to separate the walnut dreg protein. The desalination rate, protein content and recovery rate were used as indicators. Single factor experiments and response surface method were used to optimize the time used to isolate the protein from the cation exchange resin, the speed and temperature of the water bath shaker. Our results showed that the optimal time was determined at 62 min; the optimal speed of the water bath shaker was 120 rpm, and the optimal temperature was 49 °C. Under these conditions, the optimal protein recovery was 95.22% and the optimal protein content was 78.12%.
KW - Alkali-solution and acid-isolation
KW - Cation exchange resin
KW - Desalination rate
KW - Recovery rate
KW - Walnut dreg protein
UR - http://www.scopus.com/inward/record.url?scp=85094866091&partnerID=8YFLogxK
U2 - 10.1007/s11694-020-00715-7
DO - 10.1007/s11694-020-00715-7
M3 - Article
AN - SCOPUS:85094866091
SN - 2193-4126
VL - 15
SP - 1310
EP - 1316
JO - Journal of Food Measurement and Characterization
JF - Journal of Food Measurement and Characterization
IS - 2
ER -