Photoacoustic-fluorescence microendoscopy in vivo

Wenzhao Yang; Jiasheng Zhou; Weihao Shao; Myeongsu Seong; Pengbo He; Zhanhong Ye; Zhendong Guo; Lili Jing; Sung Liang Chen

doi:10.1364/OL.425753

Photoacoustic-fluorescence microendoscopy in vivo

Wenzhao Yang, Jiasheng Zhou, Weihao Shao, Myeongsu Seong, Pengbo He, Zhanhong Ye, Zhendong Guo, Lili Jing, Sung Liang Chen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

A miniature endoscope capable of imaging multiple tissue contrasts in high resolution is highly attractive, because it can provide complementary and detailed tissue information of internal organs. Here we present a photoacoustic (PA)-fluorescence (FL) endoscope for optical-resolution PA microscopy (PAM) and FL microscopy (FLM). The endoscope with a diameter of 2.8 mm achieves high lateral resolutions of 5.5 and 6.3 µm for PAM and FLM modes, respectively. In vivo imaging of zebrafish larvae and a mouse ear is conducted, and high-quality images are obtained. Additionally, in vivo endoscopic imaging of a rat rectum is demonstrated, showing the endoscopic imaging capability of our endoscope. By providing dual contrasts with high resolution, the endoscope may open up new opportunities for clinical endoscopic imaging applications.

Original language	English
Pages (from-to)	2340-2343
Number of pages	4
Journal	Optics Letters
Volume	46
Issue number	10
DOIs	https://doi.org/10.1364/OL.425753
Publication status	Published - 15 May 2021
Externally published	Yes

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title = "Photoacoustic-fluorescence microendoscopy in vivo",

abstract = "A miniature endoscope capable of imaging multiple tissue contrasts in high resolution is highly attractive, because it can provide complementary and detailed tissue information of internal organs. Here we present a photoacoustic (PA)-fluorescence (FL) endoscope for optical-resolution PA microscopy (PAM) and FL microscopy (FLM). The endoscope with a diameter of 2.8 mm achieves high lateral resolutions of 5.5 and 6.3 µm for PAM and FLM modes, respectively. In vivo imaging of zebrafish larvae and a mouse ear is conducted, and high-quality images are obtained. Additionally, in vivo endoscopic imaging of a rat rectum is demonstrated, showing the endoscopic imaging capability of our endoscope. By providing dual contrasts with high resolution, the endoscope may open up new opportunities for clinical endoscopic imaging applications.",

author = "Wenzhao Yang and Jiasheng Zhou and Weihao Shao and Myeongsu Seong and Pengbo He and Zhanhong Ye and Zhendong Guo and Lili Jing and Chen, {Sung Liang}",

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AU - Yang, Wenzhao

AU - Zhou, Jiasheng

AU - Shao, Weihao

AU - Seong, Myeongsu

AU - He, Pengbo

AU - Ye, Zhanhong

AU - Guo, Zhendong

AU - Jing, Lili

AU - Chen, Sung Liang

PY - 2021/5/15

Y1 - 2021/5/15

N2 - A miniature endoscope capable of imaging multiple tissue contrasts in high resolution is highly attractive, because it can provide complementary and detailed tissue information of internal organs. Here we present a photoacoustic (PA)-fluorescence (FL) endoscope for optical-resolution PA microscopy (PAM) and FL microscopy (FLM). The endoscope with a diameter of 2.8 mm achieves high lateral resolutions of 5.5 and 6.3 µm for PAM and FLM modes, respectively. In vivo imaging of zebrafish larvae and a mouse ear is conducted, and high-quality images are obtained. Additionally, in vivo endoscopic imaging of a rat rectum is demonstrated, showing the endoscopic imaging capability of our endoscope. By providing dual contrasts with high resolution, the endoscope may open up new opportunities for clinical endoscopic imaging applications.

AB - A miniature endoscope capable of imaging multiple tissue contrasts in high resolution is highly attractive, because it can provide complementary and detailed tissue information of internal organs. Here we present a photoacoustic (PA)-fluorescence (FL) endoscope for optical-resolution PA microscopy (PAM) and FL microscopy (FLM). The endoscope with a diameter of 2.8 mm achieves high lateral resolutions of 5.5 and 6.3 µm for PAM and FLM modes, respectively. In vivo imaging of zebrafish larvae and a mouse ear is conducted, and high-quality images are obtained. Additionally, in vivo endoscopic imaging of a rat rectum is demonstrated, showing the endoscopic imaging capability of our endoscope. By providing dual contrasts with high resolution, the endoscope may open up new opportunities for clinical endoscopic imaging applications.

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JO - Optics Letters

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Photoacoustic-fluorescence microendoscopy in vivo

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