PCR-based detection of a tracheal mite of the honey bee Acarapis woodi

Yuriko Kojima, Mikio Yoshiyama, Kiyoshi Kimura, Tatsuhiko Kadowaki*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)


The effects of the tracheal mite Acarapis woodi on the health of honey bees have been neglected since the prevalence of Varroa mites to Apis mellifera colonies. However, tracheal mite infestation of honey bee colonies still occurs worldwide and could impose negative impact on apiculture. The detection of A. woodi requires the dissection of honey bees followed by microscopic observation of the tracheal sacs. We thus developed PCR methods to detect A. woodi. These methods facilitate rapid and sensitive detection of A. woodi in many honey bee samples for epidemiologic surveys.

Original languageEnglish
Pages (from-to)135-137
Number of pages3
JournalJournal of Invertebrate Pathology
Issue number2
Publication statusPublished - Oct 2011
Externally publishedYes


  • Acarapis externus
  • Acarapis woodi
  • Honey bee
  • PCR
  • Tracheal mite

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