NGS-based high-throughput screen to identify MicroRNAs regulating growth of B-cell lymphoma

Joost Kluiver*, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, Agnieszka Dzikiewicz-Krawczyk

*Corresponding author for this work

Research output: Chapter in Book or Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)


MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages14
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • B-cell lymphoma
  • Cell growth
  • High-throughput screen
  • MiRNA inhibition
  • MiRNA overexpression
  • MicroRNA


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