Monitoring of headspace total volatile basic nitrogen from selected fish species using reflectance spectroscopic measurements of pH sensitive films

Liam Byrne*, King Tong Lau, Dermot Diamond

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)


The release of amines from decomposing fish such as trimethylamine (TMA), dimethylamine (DMA) and ammonia, collectively known as total volatile basic nitrogen (TVB-N), are in high enough concentrations in headspace to be monitored by a colour change in a pH-sensitive sensor. A method developed here uses a pH indicator dye physically trapped in a cellulose polymer film to respond to the headspace TVB-N released from selected fish species during spoilage. Two species were selected for analysis on the basis of economic importance and the levels of volatile amines released were followed with time using uv/vis reflectance spectroscopic measurements. The results show that there is a significant increase in the TVB-N content in the headspace of fish samples after an incubation period of 8-12 h for cod and 12-15 h for orange roughy.

Original languageEnglish
Pages (from-to)1338-1341
Number of pages4
Issue number10
Publication statusPublished - Oct 2002
Externally publishedYes

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