Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials

Moniek G.J.  Schmitz; Jasper G.M. Aarts; Laurence Burroughs; Phanikrishna Sudarsanam; Tim J.M. Kuijpers; Martijn Riool; Leonie de Boer; Xuan Xue; Dragan Bosnacki; Sebastian A.J. Zaat; Jan de Boer; Morgan R. Alexander; Patricia Y.W. Dankers

doi:10.1002/marc.202300638

Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials

Moniek G.J. Schmitz, Jasper G.M. Aarts, Laurence Burroughs, Phanikrishna Sudarsanam, Tim J.M. Kuijpers, Martijn Riool, Leonie de Boer, Xuan Xue, Dragan Bosnacki, Sebastian A.J. Zaat, Jan de Boer, Morgan R. Alexander, Patricia Y.W. Dankers

University of Nottingham

Research output: Contribution to journal › Article › peer-review

Abstract

A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.

Original language	English
Journal	Macromolecular Rapid Communications
DOIs	https://doi.org/10.1002/marc.202300638
Publication status	Published - 26 Mar 2024
Externally published	Yes

Keywords

antimicrobial
array
biomaterials
high throughput
screening
supramolecular
upy

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10.1002/marc.202300638

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Schmitz, M. G. J., Aarts, J. G. M., Burroughs, L., Sudarsanam, P., Kuijpers, T. J. M., Riool, M., Boer, L. D., Xue, X., Bosnacki, D., Zaat, S. A. J., Boer, J. D., Alexander, M. R., & Dankers, P. Y. W. (2024). Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials. Macromolecular Rapid Communications. https://doi.org/10.1002/marc.202300638

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title = "Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials",

abstract = "A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.",

keywords = "antimicrobial, array, biomaterials, high throughput, screening, supramolecular, upy",

author = "Schmitz, {Moniek G.J.} and Aarts, {Jasper G.M.} and Laurence Burroughs and Phanikrishna Sudarsanam and Kuijpers, {Tim J.M.} and Martijn Riool and Boer, {Leonie de} and Xuan Xue and Dragan Bosnacki and Zaat, {Sebastian A.J.} and Boer, {Jan de} and Alexander, {Morgan R.} and Dankers, {Patricia Y.W.}",

note = "Publisher Copyright: {\textcopyright} 2024 The Authors. Macromolecular Rapid Communications published by Wiley-VCH GmbH.",

year = "2024",

month = mar,

day = "26",

doi = "10.1002/marc.202300638",

language = "English",

journal = "Macromolecular Rapid Communications",

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Schmitz, MGJ, Aarts, JGM, Burroughs, L, Sudarsanam, P, Kuijpers, TJM, Riool, M, Boer, LD, Xue, X, Bosnacki, D, Zaat, SAJ, Boer, JD, Alexander, MR & Dankers, PYW 2024, 'Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials', Macromolecular Rapid Communications. https://doi.org/10.1002/marc.202300638

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T1 - Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials

AU - Schmitz, Moniek G.J.

AU - Aarts, Jasper G.M.

AU - Burroughs, Laurence

AU - Sudarsanam, Phanikrishna

AU - Kuijpers, Tim J.M.

AU - Riool, Martijn

AU - Boer, Leonie de

AU - Xue, Xuan

AU - Bosnacki, Dragan

AU - Zaat, Sebastian A.J.

AU - Boer, Jan de

AU - Alexander, Morgan R.

AU - Dankers, Patricia Y.W.

PY - 2024/3/26

Y1 - 2024/3/26

N2 - A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.

AB - A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.

KW - antimicrobial

KW - array

KW - biomaterials

KW - high throughput

KW - screening

KW - supramolecular

KW - upy

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DO - 10.1002/marc.202300638

M3 - Article

SN - 1022-1336

JO - Macromolecular Rapid Communications

JF - Macromolecular Rapid Communications

ER -

Merging Modular Molecular Design with High Throughput Screening of Cell Adhesion on Antimicrobial Supramolecular Biomaterials

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Keywords

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