Measuring endocrine (cortisol) responses of zebrafish to stress

Peter R. Canavello, Jonathan M. Cachat, Esther C. Beeson, Autumn L. Laffoon, Chelsea Grimes, Whitlee A.M. Haymore, Marco F. Elegante, Brett K. Bartels, Peter C. Hart, Salem I. Elkhayat, David H. Tien, Sopan Mohnot, Hakima Amri, Allan V. Kalueff

Research output: Chapter in Book or Report/Conference proceedingChapterpeer-review

38 Citations (Scopus)

Abstract

The zebrafish (Danio rerio) is rapidly becoming a popular model species in stress and neuroscience research. Their behavior, robustly affected by environmental and pharmacological manipulations, can be paralleled by physiological (endocrine) analysis. Zebrafish have a hypothalamic-pituitary- interrenal (HPI) axis, which is homologous to the human hypothalamic-pituitary- adrenal (HPA) axis. While mice and rats use corticosterone as their main stress hormone, both humans and zebrafish utilize cortisol. This protocol explains the whole-body cortisol extraction procedure and the use of the human salivary cortisol ELISA kit to measure the amount of cortisol in each zebrafish sample. The ability to correlate physiological data from individual fish with behavioral data provides researchers with a valuable tool for investigating stress and anxiety, and contributes to the utility of zebrafish neurobehavioral models of stress.

Original languageEnglish
Title of host publicationZebrafish Neurobehavioral Protocols
EditorsAllan Kalueff, Jonathan Cachat
Pages135-142
Number of pages8
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameNeuromethods
Volume51
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • cortisol
  • ELISA
  • HPA axis
  • HPI axis
  • physiological endpoint
  • stress
  • Zebrafish

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