Measuring endocrine (cortisol) responses of zebrafish to stress

Peter R. Canavello; Jonathan M. Cachat; Esther C. Beeson; Autumn L. Laffoon; Chelsea Grimes; Whitlee A.M. Haymore; Marco F. Elegante; Brett K. Bartels; Peter C. Hart; Salem I. Elkhayat; David H. Tien; Sopan Mohnot; Hakima Amri; Allan V. Kalueff

doi:10.1007/978-1-60761-953-6_11

Measuring endocrine (cortisol) responses of zebrafish to stress

Peter R. Canavello, Jonathan M. Cachat, Esther C. Beeson, Autumn L. Laffoon, Chelsea Grimes, Whitlee A.M. Haymore, Marco F. Elegante, Brett K. Bartels, Peter C. Hart, Salem I. Elkhayat, David H. Tien, Sopan Mohnot, Hakima Amri, Allan V. Kalueff

Research output: Chapter in Book or Report/Conference proceeding › Chapter › peer-review

39 Citations (Scopus)

Abstract

The zebrafish (Danio rerio) is rapidly becoming a popular model species in stress and neuroscience research. Their behavior, robustly affected by environmental and pharmacological manipulations, can be paralleled by physiological (endocrine) analysis. Zebrafish have a hypothalamic-pituitary- interrenal (HPI) axis, which is homologous to the human hypothalamic-pituitary- adrenal (HPA) axis. While mice and rats use corticosterone as their main stress hormone, both humans and zebrafish utilize cortisol. This protocol explains the whole-body cortisol extraction procedure and the use of the human salivary cortisol ELISA kit to measure the amount of cortisol in each zebrafish sample. The ability to correlate physiological data from individual fish with behavioral data provides researchers with a valuable tool for investigating stress and anxiety, and contributes to the utility of zebrafish neurobehavioral models of stress.

Original language	English
Title of host publication	Zebrafish Neurobehavioral Protocols
Editors	Allan Kalueff, Jonathan Cachat
Pages	135-142
Number of pages	8
DOIs	https://doi.org/10.1007/978-1-60761-953-6_11
Publication status	Published - 2011
Externally published	Yes

Publication series

Name	Neuromethods
Volume	51
ISSN (Print)	0893-2336
ISSN (Electronic)	1940-6045

Keywords

cortisol
ELISA
HPA axis
HPI axis
physiological endpoint
stress
Zebrafish

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-60761-953-6_11

Cite this

Canavello, P. R., Cachat, J. M., Beeson, E. C., Laffoon, A. L., Grimes, C., Haymore, W. A. M., Elegante, M. F., Bartels, B. K., Hart, P. C., Elkhayat, S. I., Tien, D. H., Mohnot, S., Amri, H., & Kalueff, A. V. (2011). Measuring endocrine (cortisol) responses of zebrafish to stress. In A. Kalueff, & J. Cachat (Eds.), Zebrafish Neurobehavioral Protocols (pp. 135-142). (Neuromethods; Vol. 51). https://doi.org/10.1007/978-1-60761-953-6_11

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abstract = "The zebrafish (Danio rerio) is rapidly becoming a popular model species in stress and neuroscience research. Their behavior, robustly affected by environmental and pharmacological manipulations, can be paralleled by physiological (endocrine) analysis. Zebrafish have a hypothalamic-pituitary- interrenal (HPI) axis, which is homologous to the human hypothalamic-pituitary- adrenal (HPA) axis. While mice and rats use corticosterone as their main stress hormone, both humans and zebrafish utilize cortisol. This protocol explains the whole-body cortisol extraction procedure and the use of the human salivary cortisol ELISA kit to measure the amount of cortisol in each zebrafish sample. The ability to correlate physiological data from individual fish with behavioral data provides researchers with a valuable tool for investigating stress and anxiety, and contributes to the utility of zebrafish neurobehavioral models of stress.",

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author = "Canavello, {Peter R.} and Cachat, {Jonathan M.} and Beeson, {Esther C.} and Laffoon, {Autumn L.} and Chelsea Grimes and Haymore, {Whitlee A.M.} and Elegante, {Marco F.} and Bartels, {Brett K.} and Hart, {Peter C.} and Elkhayat, {Salem I.} and Tien, {David H.} and Sopan Mohnot and Hakima Amri and Kalueff, {Allan V.}",

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doi = "10.1007/978-1-60761-953-6_11",

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Canavello, PR, Cachat, JM, Beeson, EC, Laffoon, AL, Grimes, C, Haymore, WAM, Elegante, MF, Bartels, BK, Hart, PC, Elkhayat, SI, Tien, DH, Mohnot, S, Amri, H & Kalueff, AV 2011, Measuring endocrine (cortisol) responses of zebrafish to stress. in A Kalueff & J Cachat (eds), Zebrafish Neurobehavioral Protocols. Neuromethods, vol. 51, pp. 135-142. https://doi.org/10.1007/978-1-60761-953-6_11

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AU - Cachat, Jonathan M.

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AU - Laffoon, Autumn L.

AU - Grimes, Chelsea

AU - Haymore, Whitlee A.M.

AU - Elegante, Marco F.

AU - Bartels, Brett K.

AU - Hart, Peter C.

AU - Elkhayat, Salem I.

AU - Tien, David H.

AU - Mohnot, Sopan

AU - Amri, Hakima

AU - Kalueff, Allan V.

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AB - The zebrafish (Danio rerio) is rapidly becoming a popular model species in stress and neuroscience research. Their behavior, robustly affected by environmental and pharmacological manipulations, can be paralleled by physiological (endocrine) analysis. Zebrafish have a hypothalamic-pituitary- interrenal (HPI) axis, which is homologous to the human hypothalamic-pituitary- adrenal (HPA) axis. While mice and rats use corticosterone as their main stress hormone, both humans and zebrafish utilize cortisol. This protocol explains the whole-body cortisol extraction procedure and the use of the human salivary cortisol ELISA kit to measure the amount of cortisol in each zebrafish sample. The ability to correlate physiological data from individual fish with behavioral data provides researchers with a valuable tool for investigating stress and anxiety, and contributes to the utility of zebrafish neurobehavioral models of stress.

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KW - ELISA

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ER -

Measuring endocrine (cortisol) responses of zebrafish to stress

Abstract

Publication series

Keywords

UN SDGs

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