TY - JOUR
T1 - Lactoperoxidase immobilization on silver nanoparticles enhances its antimicrobial activity
AU - Sheikh, Ishfaq Ahmad
AU - Yasir, Muhammad
AU - Khan, Imran
AU - Khan, Sher Bahadur
AU - Azum, Naveed
AU - Jiffri, Essam Hussain
AU - Kamal, Mohammad Amjad
AU - Ashraf, Ghulam Md
AU - Beg, Mohd Amin
N1 - Publisher Copyright:
© Copyright Hannah Dairy Research Foundation 2018.
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.
AB - Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.
KW - Antimicrobial activity
KW - immobilization
KW - lactoperoxidase
KW - silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85052953763&partnerID=8YFLogxK
U2 - 10.1017/S0022029918000730
DO - 10.1017/S0022029918000730
M3 - Article
C2 - 30136638
AN - SCOPUS:85052953763
SN - 0022-0299
VL - 85
SP - 460
EP - 464
JO - Journal of Dairy Research
JF - Journal of Dairy Research
IS - 4
ER -