Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants

Tatsuhiko Kadowaki, Shaoping Chen, Midori Hitomi, Erica Jacobs, Chino Kumagai, Shuang Liang, Roger Schneiter, David Singleton, Joanna Wisniewska, Alan M. Tartakoff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

147 Citations (Scopus)


To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 coming mutants, these strains do not interrupt splicing of pre-mRNA at 37°C; however four strains accumulate oversized RNA polymerase II transcripts. Some show inhibition of rRNA processing and a further subset of these strains is also characterized by inhibition of tRNA maturation. Several strains accumulate nuclear.

Original languageEnglish
Pages (from-to)649-659
Number of pages11
JournalJournal of Cell Biology
Issue number3
Publication statusPublished - Aug 1994
Externally publishedYes


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