Impulse-dependent extracellular resting dopamine concentration in rat striatum in vivo

Pan Li Zuo, Wei Yao, Liang Sun, Shu Ting Kuo, Qing Li, Shi Rong Wang, Hai Qiang Dou, Hua Dong Xu, Claire Xi Zhang, Xin Jiang Kang, Zhuan Zhou*, Bo Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


The ambient resting dopamine (DA) concentration in brain regulates cognition and motivation. Despite its importance, resting DA level in vivo remains elusive. Here, by high-frequency stimulation of the medial forebrain bundle and immediately following the stimulus-induced DA overflow, we recorded a DA "undershoot" which is a temporal reduction of DA concentration to a level below the baseline. Based on the DA undershoot, we predicted a resting DA concentration of ∼73 nM in rat striatum in vivo. Simulation studies suggested that removing basal DA by DAT during the post-stimulation inhibition of tonic DA release caused the DA undershoot, and the resting concentration of DA modulated the kinetics of the evoked DA transient. The DA undershoot was eliminated by either blocking D2 receptors with haloperidol or blocking the DA transporter (DAT) with cocaine. Therefore, the impulse-dependent resting DA concentration is in the tens of nanomolar range and is modulated by the presynaptic D2 receptors and the DAT in vivo.

Original languageEnglish
Pages (from-to)50-57
Number of pages8
JournalNeurochemistry International
Issue number1
Publication statusPublished - Jan 2013
Externally publishedYes


  • Amperometry
  • D2 receptors
  • In vivo
  • Resting dopamine
  • Striatum
  • Undershoot

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