TY - JOUR
T1 - Impact of Reduced Dietary Levels of Eicosapentaenoic Acid and Docosahexaenoic Acid on the Composition of Skin Membrane Lipids in Atlantic Salmon (Salmo salar L.)
AU - Cheng, Ken
AU - Bou, Marta
AU - Ruyter, Bente
AU - Pickova, Jana
AU - Ehtesham, Emad
AU - Du, Liang
AU - Venegas, Claudia
AU - Moazzami, Ali A.
N1 - Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/8/22
Y1 - 2018/8/22
N2 - Membrane lipids, including sphingolipids and glycerol-phospholipids, are essential in maintaining the skin's barrier function in mammals, but their composition in fish skin and their response to diets have not been evaluated. This study investigated the impacts of reducing dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on membrane lipids in the skin of Atlantic salmon through a 26 week feeding regime supplying different levels (0-2.0% of dry mass) of EPA/DHA. Ceramide, glucosylceramide, sphingomyelin, sphingosine, and sphinganine in salmon skin were analyzed for the first time. Higher concentrations of glucosylceramide and sphingomyelin and higher ratios of glucosylceramide/ceramide and sphingomyelin/ceramide were detected in the deficient group, indicating interruptions in sphingolipidomics. Changes in the glycerol-phospholipid profile in fish skin caused by reducing dietary EPA and DHA were observed. There were no dietary impacts on epidermal thickness and mucus-cell density, but the changes in the phospholipid profile suggest that low dietary EPA and DHA may interrupt the barrier function of fish skin.
AB - Membrane lipids, including sphingolipids and glycerol-phospholipids, are essential in maintaining the skin's barrier function in mammals, but their composition in fish skin and their response to diets have not been evaluated. This study investigated the impacts of reducing dietary eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on membrane lipids in the skin of Atlantic salmon through a 26 week feeding regime supplying different levels (0-2.0% of dry mass) of EPA/DHA. Ceramide, glucosylceramide, sphingomyelin, sphingosine, and sphinganine in salmon skin were analyzed for the first time. Higher concentrations of glucosylceramide and sphingomyelin and higher ratios of glucosylceramide/ceramide and sphingomyelin/ceramide were detected in the deficient group, indicating interruptions in sphingolipidomics. Changes in the glycerol-phospholipid profile in fish skin caused by reducing dietary EPA and DHA were observed. There were no dietary impacts on epidermal thickness and mucus-cell density, but the changes in the phospholipid profile suggest that low dietary EPA and DHA may interrupt the barrier function of fish skin.
KW - DHA
KW - EPA
KW - ceramide
KW - fish-skin health
KW - glycerol-phospholipids
KW - sphingolipidomics
UR - http://www.scopus.com/inward/record.url?scp=85050745229&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.8b02886
DO - 10.1021/acs.jafc.8b02886
M3 - Article
C2 - 30044633
AN - SCOPUS:85050745229
SN - 0021-8561
VL - 66
SP - 8876
EP - 8884
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 33
ER -