Global impacts of fertilization and herbivore removal on soil net nitrogen mineralization are modulated by local climate and soil properties

Anita C. Risch*, Stefan Zimmermann, Barbara Moser, Martin Schütz, Frank Hagedorn, Jennifer Firn, Philip A. Fay, Peter B. Adler, Lori A. Biederman, John M. Blair, Elizabeth T. Borer, Arthur A.D. Broadbent, Cynthia S. Brown, Marc W. Cadotte, Maria C. Caldeira, Kendi F. Davies, Augustina di Virgilio, Nico Eisenhauer, Anu Eskelinen, Johannes M.H. KnopsAndrew S. MacDougall, Rebecca L. McCulley, Brett A. Melbourne, Joslin L. Moore, Sally A. Power, Suzanne M. Prober, Eric W. Seabloom, Julia Siebert, Maria L. Silveira, Karina L. Speziale, Carly J. Stevens, Pedro M. Tognetti, Risto Virtanen, Laura Yahdjian, Raul Ochoa-Hueso

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)


Soil nitrogen (N) availability is critical for grassland functioning. However, human activities have increased the supply of biologically limiting nutrients, and changed the density and identity of mammalian herbivores. These anthropogenic changes may alter net soil N mineralization (soil net Nmin), that is, the net balance between N mineralization and immobilization, which could severely impact grassland structure and functioning. Yet, to date, little is known about how fertilization and herbivore removal individually, or jointly, affect soil net Nmin across a wide range of grasslands that vary in soil and climatic properties. Here we collected data from 22 grasslands on five continents, all part of a globally replicated experiment, to assess how fertilization and herbivore removal affected potential (laboratory-based) and realized (field-based) soil net Nmin. Herbivore removal in the absence of fertilization did not alter potential and realized soil net Nmin. However, fertilization alone and in combination with herbivore removal consistently increased potential soil net Nmin. Realized soil net Nmin, in contrast, significantly decreased in fertilized plots where herbivores were removed. Treatment effects on potential and realized soil net Nmin were contingent on site-specific soil and climatic properties. Fertilization effects on potential soil net Nmin were larger at sites with higher mean annual precipitation (MAP) and temperature of the wettest quarter (T.q.wet). Reciprocally, realized soil net Nmin declined most strongly with fertilization and herbivore removal at sites with lower MAP and higher T.q.wet. In summary, our findings show that anthropogenic nutrient enrichment, herbivore exclusion and alterations in future climatic conditions can negatively impact soil net Nmin across global grasslands under realistic field conditions. This is an important context-dependent knowledge for grassland management worldwide.

Original languageEnglish
Pages (from-to)7173-7185
Number of pages13
JournalGlobal Change Biology
Issue number12
Publication statusPublished - Dec 2020


  • NutNet
  • anthropogenic change
  • global grasslands
  • grazers
  • nitrogen
  • phosphorus
  • potassium
  • potential and realized soil net nitrogen mineralization
  • precipitation
  • temperature


Dive into the research topics of 'Global impacts of fertilization and herbivore removal on soil net nitrogen mineralization are modulated by local climate and soil properties'. Together they form a unique fingerprint.

Cite this