TY - JOUR
T1 - Furostanol Saponins from Trillium tschonoskii Promote the Expansion of Human Cord Blood Hematopoietic Stem and Progenitor Cells
AU - Wang, Bei
AU - Zhang, Jing
AU - Pang, Xu
AU - Yuan, Junyong
AU - Yang, Jie
AU - Yang, Yinjun
AU - Gao, Lin
AU - Zhang, Jie
AU - Fan, Zeng
AU - He, Lijuan
AU - Yue, Wen
AU - Li, Yanhua
AU - Pei, Xuetao
AU - Ma, Baiping
N1 - Publisher Copyright:
© 2020 American Chemical Society and American Society of Pharmacognosy
PY - 2020/9/25
Y1 - 2020/9/25
N2 - Trillium tschonoskii is a medicinal plant known to biosynthesize steroidal saponins. A phytochemical investigation of the rhizomes of T. tschonoskii led to the isolation of nine new furostanol saponins (1−9) and 11 known analogues (10−20). Five of these new compounds were shown to have hydroxy groups at the C-5 and C-6 positions, while two possess a rare aglycone containing carbonyl groups at the C-16 and C-22 positions as well as a Δ17(20) double bond, and the others have conjugated double bonds in the E-ring or have different sugar chains at the C-3 position. All the isolates were tested for their effect on the expansion of human cord blood (CB) CD34+ hematopoietic stem and progenitor cells. It was found that CB CD34+ cells treated with compounds 6, 7, 9, 10, 14, 15, and 19 showed increased numbers of rigorously phenotype-defined hematopoietic stem cells. Notably, compounds 9, 10, 13, and 14 demonstrated an enhanced ability to increase the percentages and numbers of CB CD34+CD38− cells and multipotential progenitors. The present study is the first to report that furostanol saponins from T. tschonoskii rhizomes can promote hematopoietic stem/progenitor cell (HSPC) expansion.
AB - Trillium tschonoskii is a medicinal plant known to biosynthesize steroidal saponins. A phytochemical investigation of the rhizomes of T. tschonoskii led to the isolation of nine new furostanol saponins (1−9) and 11 known analogues (10−20). Five of these new compounds were shown to have hydroxy groups at the C-5 and C-6 positions, while two possess a rare aglycone containing carbonyl groups at the C-16 and C-22 positions as well as a Δ17(20) double bond, and the others have conjugated double bonds in the E-ring or have different sugar chains at the C-3 position. All the isolates were tested for their effect on the expansion of human cord blood (CB) CD34+ hematopoietic stem and progenitor cells. It was found that CB CD34+ cells treated with compounds 6, 7, 9, 10, 14, 15, and 19 showed increased numbers of rigorously phenotype-defined hematopoietic stem cells. Notably, compounds 9, 10, 13, and 14 demonstrated an enhanced ability to increase the percentages and numbers of CB CD34+CD38− cells and multipotential progenitors. The present study is the first to report that furostanol saponins from T. tschonoskii rhizomes can promote hematopoietic stem/progenitor cell (HSPC) expansion.
UR - http://www.scopus.com/inward/record.url?scp=85091432114&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.9b01268
DO - 10.1021/acs.jnatprod.9b01268
M3 - Article
C2 - 32870000
AN - SCOPUS:85091432114
SN - 0163-3864
VL - 83
SP - 2567
EP - 2577
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 9
ER -