TY - JOUR
T1 - Folic acid-CdTe quantum dot conjugates and their applications for cancer cell targeting
AU - Suriamoorthy, Preethi
AU - Zhang, Xing
AU - Hao, Guiyang
AU - Joly, Alan G.
AU - Singh, Surya
AU - Hossu, Marius
AU - Sun, Xiankai
AU - Chen, Wei
PY - 2010/12
Y1 - 2010/12
N2 - In this study, we report the preparation, luminescence, and targeting properties of folic acid-CdTe quantum dot conjugates. Water-soluble CdTe quantum dots were synthesized and conjugated with folic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide chemistry. The influence of folic acid on the luminescence properties of CdTe quantum dots was investigated, and no energy transfer between them was observed. To investigate the efficiency of folic acid-CdTe nanoconjugates for tumor targeting, pure CdTe quantum dots and folic acid-coated CdTe quantum dots were incubated with human nasopharyngeal epidermal carcinoma cell line with positive expressing folic acid receptors (KB cells) and lung cancer cells without expression of folic acid receptors (A549 cells). For the cancer cells with positive folate receptors (KB cells), the uptake for CdTe quantum dots is very low, but for folic acid-CdTe nanoconjugates, the uptake is very high. For the lung cancer cells without folate receptors (A549 cells), the uptake for folic acid-CdTe nanoconjugates is also very low. The results indicate that folic acid is an effective targeting molecule for tumor cells with overexpressed folate receptors.
AB - In this study, we report the preparation, luminescence, and targeting properties of folic acid-CdTe quantum dot conjugates. Water-soluble CdTe quantum dots were synthesized and conjugated with folic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide chemistry. The influence of folic acid on the luminescence properties of CdTe quantum dots was investigated, and no energy transfer between them was observed. To investigate the efficiency of folic acid-CdTe nanoconjugates for tumor targeting, pure CdTe quantum dots and folic acid-coated CdTe quantum dots were incubated with human nasopharyngeal epidermal carcinoma cell line with positive expressing folic acid receptors (KB cells) and lung cancer cells without expression of folic acid receptors (A549 cells). For the cancer cells with positive folate receptors (KB cells), the uptake for CdTe quantum dots is very low, but for folic acid-CdTe nanoconjugates, the uptake is very high. For the lung cancer cells without folate receptors (A549 cells), the uptake for folic acid-CdTe nanoconjugates is also very low. The results indicate that folic acid is an effective targeting molecule for tumor cells with overexpressed folate receptors.
KW - Bioconjugation
KW - Cancer
KW - Folic acid receptors
KW - Imaging
KW - Labeling
KW - Luminescence
KW - Quantum dots
KW - Targeting
UR - http://www.scopus.com/inward/record.url?scp=79952715857&partnerID=8YFLogxK
U2 - 10.1007/s12645-010-0003-3
DO - 10.1007/s12645-010-0003-3
M3 - Article
AN - SCOPUS:79952715857
SN - 1868-6958
VL - 1
SP - 19
EP - 28
JO - Cancer Nanotechnology
JF - Cancer Nanotechnology
IS - 1-6
ER -