Effects of phthalocyanine-based molecular beacon-mediated photodynamic therapy on U251 cells

Dianshuang Xu; Xiangyu Chen; Yiru Peng; Wei Chen; Yingxin Li; Xiangyu Wang; Brian Bui; Ke'en Chen; Meng Zhou; Nobuyuki Kawai; Takashi Tamiya

doi:10.1166/nnl.2016.2218

Effects of phthalocyanine-based molecular beacon-mediated photodynamic therapy on U251 cells

Dianshuang Xu^*, Xiangyu Chen, Yiru Peng, Wei Chen, Yingxin Li, Xiangyu Wang, Brian Bui, Ke'en Chen, Meng Zhou, Nobuyuki Kawai, Takashi Tamiya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Phthalocyanine-based active photodynamic molecular beacon (PPMB) is a novel photosensitizer with a potential for treating gliomas. In this study, the anti-tumor activity of PPMB on U251 glioma cells is evaluated and the results show that the uptake of PPMB by U251 glioma cells reached a maximum after incubation for 6 h. The optimal laser dosage for PPMB-induced PDT on U251 cells is 25 J/cm² and the half-inhibitory concentration (IC50) is 3.33 μM. The cell viability-concentration curves of groups treated with PDT and PPMB are nearly identical for the treatments with and without the ambient light. The VEGF expression, ROS production, and apoptosis are increased after PDT. The HIF-1α expression after PDT is not by different from that in the control group. Therefore, it is not necessary to avoid the ambient light while using PPMB for PDT. PPMB-based PDT increased the expression of VEGF. Based on these observations, it is concluded that PPMB is an effective photosensitizer for treating U251 glioma.

Original language	English
Pages (from-to)	714-722
Number of pages	9
Journal	Nanoscience and Nanotechnology Letters
Volume	8
Issue number	9
DOIs	https://doi.org/10.1166/nnl.2016.2218
Publication status	Published - Sept 2016
Externally published	Yes

Keywords

Glioma
Molecular beacon
Photodynamic therapy
Phthalocyanine
Protease activation

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10.1166/nnl.2016.2218

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title = "Effects of phthalocyanine-based molecular beacon-mediated photodynamic therapy on U251 cells",

abstract = "Phthalocyanine-based active photodynamic molecular beacon (PPMB) is a novel photosensitizer with a potential for treating gliomas. In this study, the anti-tumor activity of PPMB on U251 glioma cells is evaluated and the results show that the uptake of PPMB by U251 glioma cells reached a maximum after incubation for 6 h. The optimal laser dosage for PPMB-induced PDT on U251 cells is 25 J/cm2 and the half-inhibitory concentration (IC50) is 3.33 μM. The cell viability-concentration curves of groups treated with PDT and PPMB are nearly identical for the treatments with and without the ambient light. The VEGF expression, ROS production, and apoptosis are increased after PDT. The HIF-1α expression after PDT is not by different from that in the control group. Therefore, it is not necessary to avoid the ambient light while using PPMB for PDT. PPMB-based PDT increased the expression of VEGF. Based on these observations, it is concluded that PPMB is an effective photosensitizer for treating U251 glioma.",

keywords = "Glioma, Molecular beacon, Photodynamic therapy, Phthalocyanine, Protease activation",

author = "Dianshuang Xu and Xiangyu Chen and Yiru Peng and Wei Chen and Yingxin Li and Xiangyu Wang and Brian Bui and Ke'en Chen and Meng Zhou and Nobuyuki Kawai and Takashi Tamiya",

year = "2016",

month = sep,

doi = "10.1166/nnl.2016.2218",

language = "English",

volume = "8",

pages = "714--722",

journal = "Nanoscience and Nanotechnology Letters",

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AU - Xu, Dianshuang

AU - Chen, Xiangyu

AU - Peng, Yiru

AU - Chen, Wei

AU - Li, Yingxin

AU - Wang, Xiangyu

AU - Bui, Brian

AU - Chen, Ke'en

AU - Zhou, Meng

AU - Kawai, Nobuyuki

AU - Tamiya, Takashi

PY - 2016/9

Y1 - 2016/9

N2 - Phthalocyanine-based active photodynamic molecular beacon (PPMB) is a novel photosensitizer with a potential for treating gliomas. In this study, the anti-tumor activity of PPMB on U251 glioma cells is evaluated and the results show that the uptake of PPMB by U251 glioma cells reached a maximum after incubation for 6 h. The optimal laser dosage for PPMB-induced PDT on U251 cells is 25 J/cm2 and the half-inhibitory concentration (IC50) is 3.33 μM. The cell viability-concentration curves of groups treated with PDT and PPMB are nearly identical for the treatments with and without the ambient light. The VEGF expression, ROS production, and apoptosis are increased after PDT. The HIF-1α expression after PDT is not by different from that in the control group. Therefore, it is not necessary to avoid the ambient light while using PPMB for PDT. PPMB-based PDT increased the expression of VEGF. Based on these observations, it is concluded that PPMB is an effective photosensitizer for treating U251 glioma.

AB - Phthalocyanine-based active photodynamic molecular beacon (PPMB) is a novel photosensitizer with a potential for treating gliomas. In this study, the anti-tumor activity of PPMB on U251 glioma cells is evaluated and the results show that the uptake of PPMB by U251 glioma cells reached a maximum after incubation for 6 h. The optimal laser dosage for PPMB-induced PDT on U251 cells is 25 J/cm2 and the half-inhibitory concentration (IC50) is 3.33 μM. The cell viability-concentration curves of groups treated with PDT and PPMB are nearly identical for the treatments with and without the ambient light. The VEGF expression, ROS production, and apoptosis are increased after PDT. The HIF-1α expression after PDT is not by different from that in the control group. Therefore, it is not necessary to avoid the ambient light while using PPMB for PDT. PPMB-based PDT increased the expression of VEGF. Based on these observations, it is concluded that PPMB is an effective photosensitizer for treating U251 glioma.

KW - Glioma

KW - Molecular beacon

KW - Photodynamic therapy

KW - Phthalocyanine

KW - Protease activation

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DO - 10.1166/nnl.2016.2218

M3 - Article

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JO - Nanoscience and Nanotechnology Letters

JF - Nanoscience and Nanotechnology Letters

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ER -

Effects of phthalocyanine-based molecular beacon-mediated photodynamic therapy on U251 cells

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Keywords

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