Abstract
Organotypic cultures of mouse and rat magnocellular neurons (MCNs) in the hypothalamo-neurohypophysial system (HNS) have served as important experimental models for the molecular and physiological study of this neuronal phenotype. However, it has been difficult to maintain significant numbers of the MCNs, particularly vasopressin MCNs, in these cultures for long periods. In this paper, we describe the use of the neurotrophic factors, leukemia inhibiting factor (LIF) and ciliary neurotrophic factor (CNTF) to rescue rat vasopressin (Avp)- and oxytocin (Oxt)-MCNs from axotomy-induced, programmed cell death in vitro. Quantitative data are presented for the efficacy of the LIF family of neurotrophic factors on the survival of MCNs in three nuclei, the paraventricular (PVN), supraoptic (SON), and accessory (ACC) nuclei in the mouse and rat hypothalamus.
Original language | English |
---|---|
Pages (from-to) | 128-133 |
Number of pages | 6 |
Journal | Journal of Neuroscience Methods |
Volume | 178 |
Issue number | 1 |
DOIs | |
Publication status | Published - 30 Mar 2009 |
Externally published | Yes |
Keywords
- CNTF
- Hypothalamus
- LIF
- Magnocellular neurons
- Neurotrophic
- Organotypic
- Oxytocin
- Vasopressin