Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals

Raju Sekar*, Longin T. Kaczmarsky, Laurie L. Richardson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

Original languageEnglish
Pages (from-to)2581-2584
Number of pages4
JournalApplied and Environmental Microbiology
Issue number8
Publication statusPublished - Apr 2009
Externally publishedYes

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