Dysregulated microRNA profile in HeLa cell lines induced by lupeol

Xiyuan Lu, Cuihong Dai, Aiju Hou, Jie Cui, Dayou Cheng, Dechang Xu*

*Corresponding author for this work

Research output: Chapter in Book or Report/Conference proceedingConference Proceedingpeer-review

1 Citation (Scopus)

Abstract

Lupeol attracted lots of research attention because of its anticancer activity. This work presents the complete microRNA profile between lupeol treated HeLa cell lines and control group, and investigates the complete small RNA sequencing data analysis process, including microRNA annotation, novel microRNA prediction, dysregulated microRNA identification and microRNA target prediction. Based on single replicate data, we applied generalized fold change (GFOLD) algorithm to detect significant regulated microRNAs. Furthermore, we adopted GOmir to predict targets of some microRNAs which have received fully attention and perform ontology analysis. The experimental results indicate that the predicted microRNAs are highly correlated with carcinogenesis.

Original languageEnglish
Title of host publicationBioinformatics Research and Applications - 10th International Symposium, ISBRA 2014, Proceedings
PublisherSpringer Verlag
Pages71-80
Number of pages10
ISBN (Print)9783319081700
DOIs
Publication statusPublished - 2014
Externally publishedYes
Event10th International Symposium on Bioinformatics Research and Applications, ISBRA 2014 - Zhangjiajie, China
Duration: 28 Jun 201430 Jun 2014

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume8492 LNBI
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Conference

Conference10th International Symposium on Bioinformatics Research and Applications, ISBRA 2014
Country/TerritoryChina
CityZhangjiajie
Period28/06/1430/06/14

Keywords

  • HeLa
  • lupeol
  • microRNA
  • small RNA sequencing

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