Disposable glucose sensors for flow injection analysis using substituted 1,4-benzoquinone mediators

King Tong Lau, Stephane A.L. De Fortescu, Lindy J. Murphy*, Jonathan M. Slater

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


The redox chemistry of several substituted benzoquinones was investigated by cyclic voltammetry at a glassy carbon electrode and candidates for inclusion in a mediated biosensor for use in flow analysis were selected on the basis of oxidation potential, electrochemical reversibility and solubility. Glucose sensors constructed by sequential deposition onto a carbon pellet of 2,6-dimethyl-1,4-benzoquinone, 2,3,5,6-tetramethyl-1,4-benzoquinone or phenyl-1,4-benzoquinone mediator solution, followed by glucose oxidase in polyvinylalcohol-Nafion solution, were tested for response to glucose using flow injection analysis. Sensors prepared from 2,6-dimethyl-1,4-benzoquinone gave highest sensitivity, with a linear range of response to glucose of 2.5-40 mM. The use of an enzyme-free comparative electrode to eliminate the response from interferents was investigated.

Original languageEnglish
Pages (from-to)975-981
Number of pages7
Issue number11
Publication statusPublished - Jul 2003
Externally publishedYes


  • Disposable
  • Electron mediator
  • Enzyme immobilization
  • FIA
  • Glucose biosensor
  • Substituted benzoquinone

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