TY - JOUR
T1 - Diffusion of nanoparticles into the capsule and cortex of a crystalline lens
AU - Schachar, Ronald A.
AU - Chen, Wei
AU - Woo, Boon K.
AU - Pierscionek, Barbara K.
AU - Zhang, Xing
AU - Ma, Lun
PY - 2008/1/16
Y1 - 2008/1/16
N2 - The purpose of this study is to determine the ability of fluorescent nanoparticles to diffuse into a crystalline lens. Intact porcine lenses from five-month-old pigs, intact human lenses obtained from three donors aged 41, 42 and 45 years, and sections of human lens cortex obtained from four donors aged 11, 19, 32, and 34 years were incubated for 72 h at 7 °C in aqueous solutions of green (566 nm) and red (652 nm) fluorescent water soluble cadmium tellurium (CdTe) nanoparticles. As demonstrated by fluorescent and confocal microscopy, the CdTe nanoparticles diffused into the porcine and human lens capsule and into human cortical lens fibres; however, the nanoparticles did not pass through the intact lens capsule. Nanoparticles can be used as a method for studying intracellular structure and biochemical pathways within the lens capsule and cortical lens fibres to further understand cataractogenesis and may serve as a carrier for chemotherapeutic agents for the potential treatment of primary and secondary cataracts.
AB - The purpose of this study is to determine the ability of fluorescent nanoparticles to diffuse into a crystalline lens. Intact porcine lenses from five-month-old pigs, intact human lenses obtained from three donors aged 41, 42 and 45 years, and sections of human lens cortex obtained from four donors aged 11, 19, 32, and 34 years were incubated for 72 h at 7 °C in aqueous solutions of green (566 nm) and red (652 nm) fluorescent water soluble cadmium tellurium (CdTe) nanoparticles. As demonstrated by fluorescent and confocal microscopy, the CdTe nanoparticles diffused into the porcine and human lens capsule and into human cortical lens fibres; however, the nanoparticles did not pass through the intact lens capsule. Nanoparticles can be used as a method for studying intracellular structure and biochemical pathways within the lens capsule and cortical lens fibres to further understand cataractogenesis and may serve as a carrier for chemotherapeutic agents for the potential treatment of primary and secondary cataracts.
UR - http://www.scopus.com/inward/record.url?scp=36849052292&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/19/02/025102
DO - 10.1088/0957-4484/19/02/025102
M3 - Article
AN - SCOPUS:36849052292
SN - 0957-4484
VL - 19
JO - Nanotechnology
JF - Nanotechnology
IS - 2
M1 - 025102
ER -