Development of a simple whole blood panel test for detection of human heart-type fatty acid-binding protein

Toshio Watanabe, Yuichi Ohkubo, Hidekazu Matsuoka, Hiroshi Kimura, Yasunori Sakai, Yasuhiko Ohkaru*, Takao Tanaka, Yasushi Kitaura

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Objective: For the diagnosis of acute myocardial infarction (AMI), we have developed a rapid and simple whole blood panel test for the detection of human heart-type fatty acid-binding protein (H-FABP) using one-step immunochromatography. Methods and Results: We have developed a whole blood panel test for rapid detection of human H-FABP using a one-step immunochromatography technique. The result of this panel test was not affected by the other contents of the blood such as bilirubin, hemoglobin and others. Furthermore, no cross-reactivity of the antibodies was found with other cardiac markers or other tissue-type FABPs. The result of this panel test was similar to the diagnostic cut-off value, 6.2 ng of H-FABP per mL of serum which was evaluated by the enzyme-linked immunosorbent assay (ELISA). Conclusion: We have developed a simple one-step immunochromatography technique to detect H-FABP in whole blood sample. Further studies are required to identify the value of this point-of-care testing (POCT) as a diagnostic marker for AMI.

Original languageEnglish
Pages (from-to)257-263
Number of pages7
JournalClinical Biochemistry
Volume34
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Acute myocardial infarction (AMI)
  • Human heart-type fatty acid-binding protein (H-FABP)
  • Immunochromatography
  • Monoclonal antibody
  • Panel test
  • Point-of-care testing (POCT)

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