TY - JOUR
T1 - Development of a simple whole blood panel test for detection of human heart-type fatty acid-binding protein
AU - Watanabe, Toshio
AU - Ohkubo, Yuichi
AU - Matsuoka, Hidekazu
AU - Kimura, Hiroshi
AU - Sakai, Yasunori
AU - Ohkaru, Yasuhiko
AU - Tanaka, Takao
AU - Kitaura, Yasushi
PY - 2001
Y1 - 2001
N2 - Objective: For the diagnosis of acute myocardial infarction (AMI), we have developed a rapid and simple whole blood panel test for the detection of human heart-type fatty acid-binding protein (H-FABP) using one-step immunochromatography. Methods and Results: We have developed a whole blood panel test for rapid detection of human H-FABP using a one-step immunochromatography technique. The result of this panel test was not affected by the other contents of the blood such as bilirubin, hemoglobin and others. Furthermore, no cross-reactivity of the antibodies was found with other cardiac markers or other tissue-type FABPs. The result of this panel test was similar to the diagnostic cut-off value, 6.2 ng of H-FABP per mL of serum which was evaluated by the enzyme-linked immunosorbent assay (ELISA). Conclusion: We have developed a simple one-step immunochromatography technique to detect H-FABP in whole blood sample. Further studies are required to identify the value of this point-of-care testing (POCT) as a diagnostic marker for AMI.
AB - Objective: For the diagnosis of acute myocardial infarction (AMI), we have developed a rapid and simple whole blood panel test for the detection of human heart-type fatty acid-binding protein (H-FABP) using one-step immunochromatography. Methods and Results: We have developed a whole blood panel test for rapid detection of human H-FABP using a one-step immunochromatography technique. The result of this panel test was not affected by the other contents of the blood such as bilirubin, hemoglobin and others. Furthermore, no cross-reactivity of the antibodies was found with other cardiac markers or other tissue-type FABPs. The result of this panel test was similar to the diagnostic cut-off value, 6.2 ng of H-FABP per mL of serum which was evaluated by the enzyme-linked immunosorbent assay (ELISA). Conclusion: We have developed a simple one-step immunochromatography technique to detect H-FABP in whole blood sample. Further studies are required to identify the value of this point-of-care testing (POCT) as a diagnostic marker for AMI.
KW - Acute myocardial infarction (AMI)
KW - Human heart-type fatty acid-binding protein (H-FABP)
KW - Immunochromatography
KW - Monoclonal antibody
KW - Panel test
KW - Point-of-care testing (POCT)
UR - http://www.scopus.com/inward/record.url?scp=0034968361&partnerID=8YFLogxK
U2 - 10.1016/S0009-9120(01)00200-4
DO - 10.1016/S0009-9120(01)00200-4
M3 - Article
C2 - 11440724
AN - SCOPUS:0034968361
SN - 0009-9120
VL - 34
SP - 257
EP - 263
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 4
ER -