TY - JOUR
T1 - Comparative study of the pesticide sensitivity of butyrylcholinesterases from chicken serum and horse serum
AU - Li, Wei
AU - He, Shao Zhi
AU - Zhang, Quan
AU - Yang, Xiao
AU - Rao, Yu
AU - Qian, Shan
AU - Chen, Xiang Gui
PY - 2014
Y1 - 2014
N2 - The inhibition effect of butyrylcholinesterase (BChE) in the chicken serum to pesticide was investigated, and new sensitive enzyme source to the quick examination of pesticide were provided. Taking the horse serum BChE for comparison, enzymology properties of the chicken serum BChE and its sensitivity to pesticides were studied by the colorimetric method of Ellman's, and then the half inhibitory concentration value (IC50) was calculated. The results showed that both BChE reached their best reactivity at 35°C and pH 8.0, no substrate inhibition effect was observed, and both were sensitive to physostigmine but relatively insensitive to donepezil HCL. The IC50 value for trichlorfon, carbofuran, methomyl, metolcarb, dichlorvos to the chicken serum BChE were 0.77, 31.99, 1.14, 5.49, 0.95 μmol/L, respectively, while the IC50 value for those five pesticides to horse serum BChE were 3.88, 1.22, 22.58, 24.18, 5.46 μmol/L, respectively. Thus, the chicken serum BChE has good sensitivity to pesticides and could be used as a candidate enzyme source for rapid detection of pesticide residues.
AB - The inhibition effect of butyrylcholinesterase (BChE) in the chicken serum to pesticide was investigated, and new sensitive enzyme source to the quick examination of pesticide were provided. Taking the horse serum BChE for comparison, enzymology properties of the chicken serum BChE and its sensitivity to pesticides were studied by the colorimetric method of Ellman's, and then the half inhibitory concentration value (IC50) was calculated. The results showed that both BChE reached their best reactivity at 35°C and pH 8.0, no substrate inhibition effect was observed, and both were sensitive to physostigmine but relatively insensitive to donepezil HCL. The IC50 value for trichlorfon, carbofuran, methomyl, metolcarb, dichlorvos to the chicken serum BChE were 0.77, 31.99, 1.14, 5.49, 0.95 μmol/L, respectively, while the IC50 value for those five pesticides to horse serum BChE were 3.88, 1.22, 22.58, 24.18, 5.46 μmol/L, respectively. Thus, the chicken serum BChE has good sensitivity to pesticides and could be used as a candidate enzyme source for rapid detection of pesticide residues.
KW - Butyrylcholinesterase
KW - Chicken serum
KW - Pesticides
KW - Sensitivity
UR - http://www.scopus.com/inward/record.url?scp=84902115979&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84902115979
SN - 1673-9078
VL - 30
SP - 69
EP - 74
JO - Modern Food Science and Technology
JF - Modern Food Science and Technology
IS - 5
ER -