Cloning, expression, and molecular dynamics simulations of a xylosidase obtained from Thermomyces lanuginosus

Vashni Gramany, Faez Iqbal Khan*, Algasan Govender, Krishna Bisetty, Suren Singh, Kugenthiren Permaul

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)


The aim of this study was to clone, express, and characterize a β-xylosidase (Tlxyn1) from the thermophilic fungus Thermomyces lanuginosus SSBP in Pichia pastoris GS115 as well as analyze optimal activity and stability using computational and experimental methods. The enzyme was constitutively expressed using the GAP promoter and secreted into the medium due to the alpha-mating factor secretion signal present on the expression vector pBGPI. The 1276 bp gene consists of an open reading frame that does not contain introns. A 12% SDS–PAGE gel revealed a major protein band at an estimated molecular mass of 50 kDa which corresponded to zymogram analysis. The three-dimensional structure of β-xylosidase was predicted, and molecular dynamics simulations at different ranges of temperature and pH were performed in order to predict optimal activity and folding energy. The results suggested a strong conformational temperature and pH dependence. The recombinant enzyme exhibited optimal activity at pH 7 and 50°C and retained 80% activity at 50°C, pH 7 for about 45 min. This is the first report of the cloning, functional expression, and simulations study of a β-xylosidase from Thermomyces species in a fungal host.

Original languageEnglish
Pages (from-to)1681-1692
Number of pages12
JournalJournal of Biomolecular Structure and Dynamics
Issue number8
Publication statusPublished - 2 Aug 2016
Externally publishedYes


  • cloning
  • expression
  • molecular dynamics
  • pK
  • structure prediction


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