Characterization of behavioral and endocrine effects of LSD on zebrafish

Leah Grossman, Eli Utterback, Adam Stewart, Siddharth Gaikwad, Kyung Min Chung, Christopher Suciu, Keith Wong, Marco Elegante, Salem Elkhayat, Julia Tan, Thomas Gilder, Nadine Wu, John DiLeo, Jonathan Cachat, Allan V. Kalueff*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

212 Citations (Scopus)

Abstract

Lysergic acid diethylamide (LSD) is a potent hallucinogenic drug that strongly affects animal and human behavior. Although adult zebrafish (Danio rerio) are emerging as a promising neurobehavioral model, the effects of LSD on zebrafish have not been investigated previously. Several behavioral paradigms (the novel tank, observation cylinder, light-dark box, open field, T-maze, social preference and shoaling tests), as well as modern video-tracking tools and whole-body cortisol assay were used to characterize the effects of acute LSD in zebrafish. While lower doses (5-100μg/L) did not affect zebrafish behavior, 250μg/L LSD increased top dwelling and reduced freezing in the novel tank and observation cylinder tests, also affecting spatiotemporal patterns of activity (as assessed by 3D reconstruction of zebrafish traces and ethograms). LSD evoked mild thigmotaxis in the open field test, increased light behavior in the light-dark test, reduced the number of arm entries and freezing in the T-maze and social preference test, without affecting social preference. In contrast, LSD affected zebrafish shoaling (increasing the inter-fish distance in a group), and elevated whole-body cortisol levels. Overall, our findings show sensitivity of zebrafish to LSD action, and support the use of zebrafish models to study hallucinogenic drugs of abuse.

Original languageEnglish
Pages (from-to)277-284
Number of pages8
JournalBehavioural Brain Research
Volume214
Issue number2
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Keywords

  • Adult zebrafish
  • Behavioral paradigms
  • Cortisol
  • Hallucinogenic drugs
  • LSD
  • Screening

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