Butelase 1: A Versatile Ligase for Peptide and Protein Macrocyclization

Giang K. T. Nguyen, Antony Kam, Shining Loo, Anna E. Jansson, Lucy X. Pan, James P. Tam*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

133 Citations (Scopus)


Macrocyclization is a valuable tool for drug design and protein engineering. Although various methods have been developed to prepare macrocycles, a general and efficient strategy is needed. Here we report a highly efficient method using butelase 1 to macrocyclize peptides and proteins ranging in sizes from 26 to >200 residues. We achieved cyclizations that are 20,000 times faster than sortase A, the most widely used ligase for protein cyclization. The reactions completed within minutes with up to 95% yields.
Original languageEnglish
Pages (from-to)15398-15401
JournalJournal of the American Chemical Society
Issue number49
Publication statusPublished - 3 Dec 2015


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