Anti-diabetic potential of Pueraria lobata root extract through promoting insulin signaling by PTP1B inhibition

Ran Sun, Xinxian Deng, Dongdong Zhang, Fangzhou Xie, Di Wang, Juntao Wang, Mojdeh S. Tavallaie, Faqin Jiang*, Lei Fu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Type 2 diabetes mellitus is a fast-growing epidemic affecting people globally. We initiated the project by searching the possible target of the Pueraria lobata root extract (P. lobata). We conducted the IC 50 assays of P. lobata on the four diabetes-related proteins: PTP1B, TCPTP, SHP-2 and DPP-4. Results indicated that P. lobata exhibited high PTP1B inhibitory activity with IC 50 of 0.043 mg/ml. Treated insulin-resistant HepG2 cells with 0.0115 mg/ml of P. lobata increased the glucose uptake by two times compared with the negative control. Further, we performed OGTT test on the diabetic C57BL/6 male mice. 20% decreased blood glucose (AUC) was obtained with a dose of 1 g/kg P. lobata compared with the negative control. Herein, we were able to demonstrate the antidiabetic effects of P. lobata might be related to the inhibition of PTP1B and therefore, bettering the insulin signaling pathway.

Original languageEnglish
Pages (from-to)12-15
Number of pages4
JournalBioorganic Chemistry
Volume87
DOIs
Publication statusPublished - Jun 2019
Externally publishedYes

Keywords

  • Anti-diabetic activities
  • PTP1B
  • Pueraria lobata
  • T2DM

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