TY - JOUR
T1 - Anti-diabetic potential of Pueraria lobata root extract through promoting insulin signaling by PTP1B inhibition
AU - Sun, Ran
AU - Deng, Xinxian
AU - Zhang, Dongdong
AU - Xie, Fangzhou
AU - Wang, Di
AU - Wang, Juntao
AU - Tavallaie, Mojdeh S.
AU - Jiang, Faqin
AU - Fu, Lei
N1 - Publisher Copyright:
© 2019
PY - 2019/6
Y1 - 2019/6
N2 - Type 2 diabetes mellitus is a fast-growing epidemic affecting people globally. We initiated the project by searching the possible target of the Pueraria lobata root extract (P. lobata). We conducted the IC 50 assays of P. lobata on the four diabetes-related proteins: PTP1B, TCPTP, SHP-2 and DPP-4. Results indicated that P. lobata exhibited high PTP1B inhibitory activity with IC 50 of 0.043 mg/ml. Treated insulin-resistant HepG2 cells with 0.0115 mg/ml of P. lobata increased the glucose uptake by two times compared with the negative control. Further, we performed OGTT test on the diabetic C57BL/6 male mice. 20% decreased blood glucose (AUC) was obtained with a dose of 1 g/kg P. lobata compared with the negative control. Herein, we were able to demonstrate the antidiabetic effects of P. lobata might be related to the inhibition of PTP1B and therefore, bettering the insulin signaling pathway.
AB - Type 2 diabetes mellitus is a fast-growing epidemic affecting people globally. We initiated the project by searching the possible target of the Pueraria lobata root extract (P. lobata). We conducted the IC 50 assays of P. lobata on the four diabetes-related proteins: PTP1B, TCPTP, SHP-2 and DPP-4. Results indicated that P. lobata exhibited high PTP1B inhibitory activity with IC 50 of 0.043 mg/ml. Treated insulin-resistant HepG2 cells with 0.0115 mg/ml of P. lobata increased the glucose uptake by two times compared with the negative control. Further, we performed OGTT test on the diabetic C57BL/6 male mice. 20% decreased blood glucose (AUC) was obtained with a dose of 1 g/kg P. lobata compared with the negative control. Herein, we were able to demonstrate the antidiabetic effects of P. lobata might be related to the inhibition of PTP1B and therefore, bettering the insulin signaling pathway.
KW - Anti-diabetic activities
KW - PTP1B
KW - Pueraria lobata
KW - T2DM
UR - http://www.scopus.com/inward/record.url?scp=85062426530&partnerID=8YFLogxK
U2 - 10.1016/j.bioorg.2019.02.046
DO - 10.1016/j.bioorg.2019.02.046
M3 - Article
C2 - 30852232
AN - SCOPUS:85062426530
SN - 0045-2068
VL - 87
SP - 12
EP - 15
JO - Bioorganic Chemistry
JF - Bioorganic Chemistry
ER -
