TY - JOUR
T1 - Acetotrophic methanogens are sensitive to long-term nickel contamination in paddy soil
AU - Xueping, Chen
AU - Juan, Yu
AU - Zheng, Chen
AU - Hongmei, Zhang
AU - Wangda, Cheng
AU - Fayan, Bai
AU - Yu, Zheng
AU - Imran Ahamed, Khan
AU - Chiquan, He
AU - Xiaoyan, Liu
N1 - Publisher Copyright:
© 2020 The Royal Society of Chemistry.
PY - 2020/4
Y1 - 2020/4
N2 - Paddy soil accounts for approximately one-fifth of the world's cultivated area and faces a serious threat from nickel (Ni). Ni pollution has an impact on the activity, composition and emission of methanogens in paddy, which is a major natural source of methane (CH4) emissions. We combined a high-throughput sequencing approach and laboratory incubation methods to evaluate the impact of long-term Ni pollution on the methanogenic archaeal community in paddy soil. The highest rate of CH4 production was 697 mg kg-1 of dry soil per d with the addition of sodium acetate at 50 mg kg-1 of Ni, which was significantly negatively correlated with the total and available Ni (p < 0.05). While the highest CH4 production rates were 485 and 544 mg kg-1 of dry soil per d with the addition of sodium formate and methanol, respectively, there was no significant difference in the CH4 production rate and maximum CH4 accumulation between the different Ni additions. Heavy pollution with 500 mg kg-1 of Ni unexceptionally inhibited the relative abundance of various genera of methanogens (22.2% in total). The abundance of acetotrophic Methanosaeta decreased with an increasing concentration of Ni (3.25-1.11%). The diverse nutrient types of species belonging to Methanosarcina were the highest under treatment with Ni200 (18.0%), and lowest in the soil with 500 mg kg-1 of Ni (2.8%). Similarly, the abundances of the most abundant hydrogenotrophic methanogens of Methanocellales were relatively high with Ni200 (26.2%) compared with those with Ni500 (5.4%). The mcrA gene was enriched under the light pollution treatment (50 mg kg-1 of Ni, 6.73 × 107 ± 9.0 × 106 copies per g of soil) compared with the control (4.18 × 107 ± 5.1 × 106 copies per g of soil). These results indicate that the long-term pollution by Ni has an impact on the activity and composition of methanogens with heavy Ni pollution, and in particular, acetotrophic methanogens are sensitive to Ni pollution in paddy soil.
AB - Paddy soil accounts for approximately one-fifth of the world's cultivated area and faces a serious threat from nickel (Ni). Ni pollution has an impact on the activity, composition and emission of methanogens in paddy, which is a major natural source of methane (CH4) emissions. We combined a high-throughput sequencing approach and laboratory incubation methods to evaluate the impact of long-term Ni pollution on the methanogenic archaeal community in paddy soil. The highest rate of CH4 production was 697 mg kg-1 of dry soil per d with the addition of sodium acetate at 50 mg kg-1 of Ni, which was significantly negatively correlated with the total and available Ni (p < 0.05). While the highest CH4 production rates were 485 and 544 mg kg-1 of dry soil per d with the addition of sodium formate and methanol, respectively, there was no significant difference in the CH4 production rate and maximum CH4 accumulation between the different Ni additions. Heavy pollution with 500 mg kg-1 of Ni unexceptionally inhibited the relative abundance of various genera of methanogens (22.2% in total). The abundance of acetotrophic Methanosaeta decreased with an increasing concentration of Ni (3.25-1.11%). The diverse nutrient types of species belonging to Methanosarcina were the highest under treatment with Ni200 (18.0%), and lowest in the soil with 500 mg kg-1 of Ni (2.8%). Similarly, the abundances of the most abundant hydrogenotrophic methanogens of Methanocellales were relatively high with Ni200 (26.2%) compared with those with Ni500 (5.4%). The mcrA gene was enriched under the light pollution treatment (50 mg kg-1 of Ni, 6.73 × 107 ± 9.0 × 106 copies per g of soil) compared with the control (4.18 × 107 ± 5.1 × 106 copies per g of soil). These results indicate that the long-term pollution by Ni has an impact on the activity and composition of methanogens with heavy Ni pollution, and in particular, acetotrophic methanogens are sensitive to Ni pollution in paddy soil.
UR - http://www.scopus.com/inward/record.url?scp=85084184954&partnerID=8YFLogxK
U2 - 10.1039/d0em00029a
DO - 10.1039/d0em00029a
M3 - Article
C2 - 32096538
AN - SCOPUS:85084184954
SN - 2050-7887
VL - 22
SP - 1014
EP - 1025
JO - Environmental Science: Processes and Impacts
JF - Environmental Science: Processes and Impacts
IS - 4
ER -