A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

Pengfei Song; Xianke Dong; Xinyu Liu

doi:10.1109/TRANSDUCERS.2015.7181009

A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

Pengfei Song, Xianke Dong, Xinyu Liu

McGill University

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

2 Citations (Scopus)

Abstract

This paper reports a novel microfluidic worm immobilization device that enables, for the first time, automated, high-speed microinjection of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with an automated robotic system, is capable of sequentially loading, immobilizing, injecting, and sorting C. elegans with minimal human intervention. Using the device, we demonstrate an injection speed of 6.6 worm/min and a pre-sorting success rate of 77.5% (post-sorting: 100%), both much higher than the performance of manual operation (speed: 1 worm per 4 min; success rate: ∼30%).

Original language	English
Title of host publication	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	658-661
Number of pages	4
ISBN (Electronic)	9781479989553
DOIs	https://doi.org/10.1109/TRANSDUCERS.2015.7181009
Publication status	Published - 5 Aug 2015
Externally published	Yes
Event	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 - Anchorage, United States Duration: 21 Jun 2015 → 25 Jun 2015

Publication series

Name	2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Conference

Conference	18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015
Country/Territory	United States
City	Anchorage
Period	21/06/15 → 25/06/15

Keywords

C. elegans
automation
drug delivery
gene Screening
microfluidics
microinjection

Access to Document

10.1109/TRANSDUCERS.2015.7181009

Cite this

Song, P., Dong, X., & Liu, X. (2015). A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 (pp. 658-661). Article 7181009 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2015.7181009

Song, Pengfei ; Dong, Xianke ; Liu, Xinyu. / A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans. 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. pp. 658-661 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

@inproceedings{2e4aa8ea899742e8b9908c6215270e18,

title = "A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans",

abstract = "This paper reports a novel microfluidic worm immobilization device that enables, for the first time, automated, high-speed microinjection of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with an automated robotic system, is capable of sequentially loading, immobilizing, injecting, and sorting C. elegans with minimal human intervention. Using the device, we demonstrate an injection speed of 6.6 worm/min and a pre-sorting success rate of 77.5% (post-sorting: 100%), both much higher than the performance of manual operation (speed: 1 worm per 4 min; success rate: ∼30%).",

keywords = "C. elegans, automation, drug delivery, gene Screening, microfluidics, microinjection",

author = "Pengfei Song and Xianke Dong and Xinyu Liu",

note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015 ; Conference date: 21-06-2015 Through 25-06-2015",

year = "2015",

month = aug,

day = "5",

doi = "10.1109/TRANSDUCERS.2015.7181009",

language = "English",

series = "2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "658--661",

booktitle = "2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015",

}

Song, P, Dong, X & Liu, X 2015, A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans. in 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015., 7181009, 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Institute of Electrical and Electronics Engineers Inc., pp. 658-661, 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015, Anchorage, United States, 21/06/15. https://doi.org/10.1109/TRANSDUCERS.2015.7181009

A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans. / Song, Pengfei; Dong, Xianke; Liu, Xinyu.
2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc., 2015. p. 658-661 7181009 (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015).

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

TY - GEN

T1 - A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

AU - Song, Pengfei

AU - Dong, Xianke

AU - Liu, Xinyu

PY - 2015/8/5

Y1 - 2015/8/5

N2 - This paper reports a novel microfluidic worm immobilization device that enables, for the first time, automated, high-speed microinjection of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with an automated robotic system, is capable of sequentially loading, immobilizing, injecting, and sorting C. elegans with minimal human intervention. Using the device, we demonstrate an injection speed of 6.6 worm/min and a pre-sorting success rate of 77.5% (post-sorting: 100%), both much higher than the performance of manual operation (speed: 1 worm per 4 min; success rate: ∼30%).

AB - This paper reports a novel microfluidic worm immobilization device that enables, for the first time, automated, high-speed microinjection of the nematode worm Caenorhabditis elegans. The microfluidic device, integrated with an automated robotic system, is capable of sequentially loading, immobilizing, injecting, and sorting C. elegans with minimal human intervention. Using the device, we demonstrate an injection speed of 6.6 worm/min and a pre-sorting success rate of 77.5% (post-sorting: 100%), both much higher than the performance of manual operation (speed: 1 worm per 4 min; success rate: ∼30%).

KW - C. elegans

KW - automation

KW - drug delivery

KW - gene Screening

KW - microfluidics

KW - microinjection

UR - http://www.scopus.com/inward/record.url?scp=84955479267&partnerID=8YFLogxK

U2 - 10.1109/TRANSDUCERS.2015.7181009

DO - 10.1109/TRANSDUCERS.2015.7181009

M3 - Conference Proceeding

AN - SCOPUS:84955479267

T3 - 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

SP - 658

EP - 661

BT - 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015

Y2 - 21 June 2015 through 25 June 2015

ER -

Song P, Dong X, Liu X. A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans. In 2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015. Institute of Electrical and Electronics Engineers Inc. 2015. p. 658-661. 7181009. (2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015). doi: 10.1109/TRANSDUCERS.2015.7181009

A microfluidic device for automated, high-speed microinjection of Caenorhabditis elegans

Abstract

Publication series

Conference

Keywords

Access to Document

Other files and links

Cite this