A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties

Ruipu Xin, Suijian Qi, Chaoxi Zeng, Faez Iqbal Khan, Bo Yang, Yonghua Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

96 Citations (Scopus)

Abstract

In this study, the natural deep eutectic solvents (NADESs) based on trehalose and choline chloride have been prepared to enhance the protein thermostability. The results of fourier transform infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy suggested that there were intensive hydrogen-bonding interactions between trehalose and choline chloride in TCCL3-DES and TCCL3-DES75. The physicochemical properties of TCCL3-DES and TCCL3-DES75 were investigated in the temperature range of 293.15–363.15 K. Our results revealed that the thermostability of lysozyme, a model protein used in this study was dramatically increased in TCCL3-DES75, as evidenced by the disappearance of the denaturing peak from their Differential Scanning Calorimetry (DSC) traces. The results of circular dichroism (CD) experiments further demonstrated that the lysozyme in TCCL3-DES75 unfolded partially at 90 °C and recovered to the initial structure at 20 °C. The study suggests that TCCL3-DES75 might be a potential solvent for stabilizing proteins.

Original languageEnglish
Pages (from-to)560-567
Number of pages8
JournalFood Chemistry
Volume217
DOIs
Publication statusPublished - 15 Feb 2017
Externally publishedYes

Keywords

  • Choline chloride
  • Deep eutectic solvents
  • Lysozyme
  • Physicochemical properties
  • Thermostability
  • Trehalose

Fingerprint

Dive into the research topics of 'A functional natural deep eutectic solvent based on trehalose: Structural and physicochemical properties'. Together they form a unique fingerprint.

Cite this