Chromatin Nanofiber displaying Cell-penetrating peptides for DNA Vaccine Application

Activity: SupervisionMaster Dissertation Supervision


Cancer has been extensively studied to develop better therapeutics, however current treatments are still considered to be less-effective, leading to the poor prognosis. Recently, DNA vaccine has been one of promising cancer treatments by inducing systemic anti-tumor immune responses with little side effects. However, DNA vaccine should overcome some barriers for the clinical applications that requires effective DNA delivery to cell nuclei and cell/tissue-specific targeting. Here, we aim to examine a DNA vaccine delivery system using cell penetration peptide-displaying chromatin. In this study, the histone genes were engineered to fuse either Trans-activator of transcription (TAT) peptide derived from Human immunodeficiency virus (HIV) or fusogenic region of glycoprotein H peptide from Herpes simplex virus (HSV) at the N terminus. Individual histones and octamers were prepared for chromatin assembly in vitro using plasmids. Our results showed high penetration efficiency of HSV gH peptide-fused histones and possible nuclear localization evidence of gH modified chromatins. The studies suggest that chromatin can be a useful platform for a potential DNA vaccine delivery application, which deem to explore further tumor antigen gene expressions in tissue culture models and animal studies.
Period1 Sept 202131 May 2023
ExamineeTatsuhiko Kadowaki & Jeong Park
Degree of RecognitionInternational


  • DNA vaccine delivery