The role of mirnas in cisplatin-resistant hela cells

Yubo Yang; Cuihong Dai; Zhipeng Cai; Aiju Hou; Dayou Cheng; Dechang Xu

doi:10.1007/978-3-319-19048-8_30

The role of mirnas in cisplatin-resistant hela cells

Yubo Yang
, Cuihong Dai
, Zhipeng Cai
, Aiju Hou
, Dayou Cheng
, Dechang Xu^*

^*Corresponding author for this work

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

1 Citation (Scopus)

Abstract

Chemotherapy is the main strategy in the treatment of cancer, however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 and HeLa/CR4). According to the previous studies and exiting evidence, we selected five miRNAs (miR-183, miR-182, miR-30a, miR-15b and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was used to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines while miR-30a was significantly down-regulated. At the same time, the targets they regulated are related to the drug-resistance. The expression alteration of selected miRNAs in resistant cell lines compared to their parent HeLa cell line suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy.

Original language	English
Title of host publication	Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings
Editors	Robert Harrison, Yaohang Li, Ion Măndoiu
Publisher	Springer Verlag
Pages	355-365
Number of pages	11
ISBN (Print)	9783319190471
DOIs	https://doi.org/10.1007/978-3-319-19048-8_30
Publication status	Published - 2015
Externally published	Yes
Event	11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015 - Norfolk, United States Duration: 7 Jun 2015 → 10 Jun 2015

Publication series

Name	Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume	9096
ISSN (Print)	0302-9743
ISSN (Electronic)	1611-3349

Conference

Conference	11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015
Country/Territory	United States
City	Norfolk
Period	7/06/15 → 10/06/15

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Chemotherapy
Cisplatin-resistance
HeLa cell
MiRNAs

Access to Document

10.1007/978-3-319-19048-8_30

Cite this

Yang, Y., Dai, C., Cai, Z., Hou, A., Cheng, D., & Xu, D. (2015). The role of mirnas in cisplatin-resistant hela cells. In R. Harrison, Y. Li, & I. Măndoiu (Eds.), Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings (pp. 355-365). (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 9096). Springer Verlag. https://doi.org/10.1007/978-3-319-19048-8_30

Yang, Yubo ; Dai, Cuihong ; Cai, Zhipeng et al. / The role of mirnas in cisplatin-resistant hela cells. Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings. editor / Robert Harrison ; Yaohang Li ; Ion Măndoiu. Springer Verlag, 2015. pp. 355-365 (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)).

@inproceedings{83c1d2947fc242dd8937e666420f77cb,

title = "The role of mirnas in cisplatin-resistant hela cells",

abstract = "Chemotherapy is the main strategy in the treatment of cancer, however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 and HeLa/CR4). According to the previous studies and exiting evidence, we selected five miRNAs (miR-183, miR-182, miR-30a, miR-15b and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was used to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines while miR-30a was significantly down-regulated. At the same time, the targets they regulated are related to the drug-resistance. The expression alteration of selected miRNAs in resistant cell lines compared to their parent HeLa cell line suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy.",

keywords = "Chemotherapy, Cisplatin-resistance, HeLa cell, MiRNAs",

author = "Yubo Yang and Cuihong Dai and Zhipeng Cai and Aiju Hou and Dayou Cheng and Dechang Xu",

note = "Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2015.; 11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015 ; Conference date: 07-06-2015 Through 10-06-2015",

year = "2015",

doi = "10.1007/978-3-319-19048-8\_30",

language = "English",

isbn = "9783319190471",

series = "Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)",

publisher = "Springer Verlag",

pages = "355--365",

editor = "Robert Harrison and Yaohang Li and Ion M{\u a}ndoiu",

booktitle = "Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings",

}

Yang, Y, Dai, C, Cai, Z, Hou, A, Cheng, D & Xu, D 2015, The role of mirnas in cisplatin-resistant hela cells. in R Harrison, Y Li & I Măndoiu (eds), Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings. Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics), vol. 9096, Springer Verlag, pp. 355-365, 11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015, Norfolk, United States, 7/06/15. https://doi.org/10.1007/978-3-319-19048-8_30

The role of mirnas in cisplatin-resistant hela cells. / Yang, Yubo; Dai, Cuihong; Cai, Zhipeng et al.
Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings. ed. / Robert Harrison; Yaohang Li; Ion Măndoiu. Springer Verlag, 2015. p. 355-365 (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 9096).

Research output: Chapter in Book or Report/Conference proceeding › Conference Proceeding › peer-review

TY - GEN

T1 - The role of mirnas in cisplatin-resistant hela cells

AU - Yang, Yubo

AU - Dai, Cuihong

AU - Cai, Zhipeng

AU - Hou, Aiju

AU - Cheng, Dayou

AU - Xu, Dechang

N1 - Publisher Copyright: © Springer International Publishing Switzerland 2015.

PY - 2015

Y1 - 2015

N2 - Chemotherapy is the main strategy in the treatment of cancer, however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 and HeLa/CR4). According to the previous studies and exiting evidence, we selected five miRNAs (miR-183, miR-182, miR-30a, miR-15b and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was used to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines while miR-30a was significantly down-regulated. At the same time, the targets they regulated are related to the drug-resistance. The expression alteration of selected miRNAs in resistant cell lines compared to their parent HeLa cell line suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy.

AB - Chemotherapy is the main strategy in the treatment of cancer, however, the development of drug-resistance is the obstacle in long-term treatment of cervical cancer. Cisplatin is one of the most common drugs used in cancer therapy. Recently, accumulating evidence suggests that miRNAs are involved in various bioactivities in oncogenesis. It is not unexpected that miRNAs play a key role in acquiring of drug-resistance in the progression of tumor. In this study, we induced and maintained four levels of cisplatin-resistant HeLa cell lines (HeLa/CR1, HeLa/CR2, HeLa/CR3 and HeLa/CR4). According to the previous studies and exiting evidence, we selected five miRNAs (miR-183, miR-182, miR-30a, miR-15b and miR-16) and their potential target mRNAs as our research targets. The real-time RT-PCR was used to detect the relative expression of miRNAs and their mRNAs. The results show that miR-182 and miR-15b were up-regulated in resistant cell lines while miR-30a was significantly down-regulated. At the same time, the targets they regulated are related to the drug-resistance. The expression alteration of selected miRNAs in resistant cell lines compared to their parent HeLa cell line suggests that HeLa cell drug resistance is associated with distinct miRNAs, which indicates that miRNAs may be one of the therapy targets in the treatment of cervical cancer by sensitizing cell to chemotherapy.

KW - Chemotherapy

KW - Cisplatin-resistance

KW - HeLa cell

KW - MiRNAs

UR - https://www.scopus.com/pages/publications/84944583979

U2 - 10.1007/978-3-319-19048-8_30

DO - 10.1007/978-3-319-19048-8_30

M3 - Conference Proceeding

AN - SCOPUS:84944583979

SN - 9783319190471

T3 - Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)

SP - 355

EP - 365

BT - Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings

A2 - Harrison, Robert

A2 - Li, Yaohang

A2 - Măndoiu, Ion

PB - Springer Verlag

T2 - 11th International Symposium on Bioinformatics Research and Applications, ISBRA 2015

Y2 - 7 June 2015 through 10 June 2015

ER -

Yang Y, Dai C, Cai Z, Hou A, Cheng D, Xu D. The role of mirnas in cisplatin-resistant hela cells. In Harrison R, Li Y, Măndoiu I, editors, Bioinformatics Research and Applications - 11th International Symposium, ISBRA 2015, Proceedings. Springer Verlag. 2015. p. 355-365. (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)). doi: 10.1007/978-3-319-19048-8_30

The role of mirnas in cisplatin-resistant hela cells

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this