Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells

Xian  Zhou; Valentina Razmovski-Naumovski; Antony Kam; Dennis Chang; Chun Guang Li; Kelvin  Chan; Alan Bensoussan

Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells

Xian Zhou
, Valentina Razmovski-Naumovski
, Antony Kam
, Dennis Chang
, Chun Guang Li
, Kelvin Chan^*
, Alan Bensoussan

^*Corresponding author for this work

Western Sydney University
University of New South Wales
Nanyang Technological University
Liverpool John Moores University

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Background
This study investigated the protective effects of the Danshen (DS) and Sanqi (SQ) herb pair on cell survival in the human cardiovascular endothelial (EA.hy926) cell line exposed to injury.

Methods
Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their protective effects in the EA.hy926 cell line against two different cellular impairments induced by DL-homocysteine (Hcy) – adenosine (Ado) – tumour necrosis factors (TNF) and oxidative stress (H2O2), respectively. The type of interaction (synergistic, antagonistic, additive) between DS and SQ was analysed using a combination index (CI) model. The effects of key bioactive compounds from DS and SQ were tested using the same models. The compound from each herb that demonstrated the most potent activity in cell viability was combined to evaluate their synergistic/antagonistic interaction using CI.

Results
DS-SQ ratios of 6:4 (50–300 μg/mL) produced synergistic effects (CI
Conclusions
This study provides scientific evidence to support the traditional use of the DS-SQ combination on protecting endothelial cells through their synergistic interactions.

Original language	English
Article number	50
Journal	BMC Complementary and Alternative Medicine
Volume	19
Publication status	Published - 21 Feb 2019

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https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2458-z

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Zhou, X., Razmovski-Naumovski, V., Kam, A., Chang, D., Li, C. G., Chan, K., & Bensoussan, A. (2019). Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells. BMC Complementary and Alternative Medicine, 19, Article 50. https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2458-z

@article{35e8671035b047c294176b823200d241,

title = "Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells",

abstract = "Background This study investigated the protective effects of the Danshen (DS) and Sanqi (SQ) herb pair on cell survival in the human cardiovascular endothelial (EA.hy926) cell line exposed to injury. Methods Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their protective effects in the EA.hy926 cell line against two different cellular impairments induced by DL-homocysteine (Hcy) {\textendash} adenosine (Ado) {\textendash} tumour necrosis factors (TNF) and oxidative stress (H2O2), respectively. The type of interaction (synergistic, antagonistic, additive) between DS and SQ was analysed using a combination index (CI) model. The effects of key bioactive compounds from DS and SQ were tested using the same models. The compound from each herb that demonstrated the most potent activity in cell viability was combined to evaluate their synergistic/antagonistic interaction using CI. Results DS-SQ ratios of 6:4 (50{\textendash}300 μg/mL) produced synergistic effects (CI Conclusions This study provides scientific evidence to support the traditional use of the DS-SQ combination on protecting endothelial cells through their synergistic interactions.",

author = "Xian Zhou and Valentina Razmovski-Naumovski and Antony Kam and Dennis Chang and Li, \{Chun Guang\} and Kelvin Chan and Alan Bensoussan",

year = "2019",

month = feb,

day = "21",

language = "English",

volume = "19",

journal = "BMC Complementary and Alternative Medicine",

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Zhou, X, Razmovski-Naumovski, V, Kam, A, Chang, D, Li, CG, Chan, K & Bensoussan, A 2019, 'Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells', BMC Complementary and Alternative Medicine, vol. 19, 50. <https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-019-2458-z>

TY - JOUR

T1 - Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells

AU - Zhou, Xian

AU - Razmovski-Naumovski, Valentina

AU - Kam, Antony

AU - Chang, Dennis

AU - Li, Chun Guang

AU - Chan, Kelvin

AU - Bensoussan, Alan

PY - 2019/2/21

Y1 - 2019/2/21

N2 - Background This study investigated the protective effects of the Danshen (DS) and Sanqi (SQ) herb pair on cell survival in the human cardiovascular endothelial (EA.hy926) cell line exposed to injury. Methods Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their protective effects in the EA.hy926 cell line against two different cellular impairments induced by DL-homocysteine (Hcy) – adenosine (Ado) – tumour necrosis factors (TNF) and oxidative stress (H2O2), respectively. The type of interaction (synergistic, antagonistic, additive) between DS and SQ was analysed using a combination index (CI) model. The effects of key bioactive compounds from DS and SQ were tested using the same models. The compound from each herb that demonstrated the most potent activity in cell viability was combined to evaluate their synergistic/antagonistic interaction using CI. Results DS-SQ ratios of 6:4 (50–300 μg/mL) produced synergistic effects (CI Conclusions This study provides scientific evidence to support the traditional use of the DS-SQ combination on protecting endothelial cells through their synergistic interactions.

AB - Background This study investigated the protective effects of the Danshen (DS) and Sanqi (SQ) herb pair on cell survival in the human cardiovascular endothelial (EA.hy926) cell line exposed to injury. Methods Nine combination ratios of Danshen-Sanqi extracts (DS-SQ) were screened for their protective effects in the EA.hy926 cell line against two different cellular impairments induced by DL-homocysteine (Hcy) – adenosine (Ado) – tumour necrosis factors (TNF) and oxidative stress (H2O2), respectively. The type of interaction (synergistic, antagonistic, additive) between DS and SQ was analysed using a combination index (CI) model. The effects of key bioactive compounds from DS and SQ were tested using the same models. The compound from each herb that demonstrated the most potent activity in cell viability was combined to evaluate their synergistic/antagonistic interaction using CI. Results DS-SQ ratios of 6:4 (50–300 μg/mL) produced synergistic effects (CI Conclusions This study provides scientific evidence to support the traditional use of the DS-SQ combination on protecting endothelial cells through their synergistic interactions.

M3 - Article

C2 - 30791910

VL - 19

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

M1 - 50

ER -

Synergistic study of a Danshen (Salvia Miltiorrhizae Radix et Rhizoma) and Sanqi (Notoginseng Radix et Rhizoma) combination on cell survival in EA.hy926 cells

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