Rapid establishment of p-nitrophenol biodegradation in acetate-fed aerobic granular sludge

Venkata Nancharaiah Yarlagadda; Renu Kadali; Naresh Sharma; Raju Sekar; Venugopalan Vayalam Purath

doi:10.1007/s12010-011-9509-3

Rapid establishment of p-nitrophenol biodegradation in acetate-fed aerobic granular sludge

Venkata Nancharaiah Yarlagadda^*
, Renu Kadali
, Naresh Sharma
, Raju Sekar
, Venugopalan Vayalam Purath

^*Corresponding author for this work

Bhabha Atomic Research Centre
Xi'an Jiaotong-Liverpool University
Vellore Institute of Technology
Indian Institute of Technology Madras

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h ^-1 g ^-1 granular biomass at an initial PNP concentration of 50 mg L ^-1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.

Original language	English
Pages (from-to)	1225-1235
Number of pages	11
Journal	Applied Biochemistry and Biotechnology
Volume	166
Issue number	5
DOIs	https://doi.org/10.1007/s12010-011-9509-3
Publication status	Published - Mar 2012

Keywords

Aerobic granular sludge
Aerobic granules
Biodegradation
p-nitrophenol

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title = "Rapid establishment of p-nitrophenol biodegradation in acetate-fed aerobic granular sludge",

abstract = "The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h -1 g -1 granular biomass at an initial PNP concentration of 50 mg L -1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.",

keywords = "Aerobic granular sludge, Aerobic granules, Biodegradation, p-nitrophenol",

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doi = "10.1007/s12010-011-9509-3",

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AU - Yarlagadda, Venkata Nancharaiah

AU - Kadali, Renu

AU - Sharma, Naresh

AU - Sekar, Raju

AU - Purath, Venugopalan Vayalam

PY - 2012/3

Y1 - 2012/3

N2 - The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h -1 g -1 granular biomass at an initial PNP concentration of 50 mg L -1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.

AB - The aim of the study was to investigate the acclimation of precultivated acetatefed aerobic granular sludge to a toxic xenobiotic biodegradation. Establishment of p-nitrophenol (PNP) biodegradation in acetate-fed aerobic granular sludge and concomitant changes in the microstructure and bacterial community were determined. Rapid establishment of PNP utilization was observed in the granular sludge when fed with PNP as the sole carbon source. The specific PNP removal was 36-mg h -1 g -1 granular biomass at an initial PNP concentration of 50 mg L -1. The presence of PNP resulted in significant membrane damage in a subpopulation of the bacterial consortium, as shown by BacLight viability staining. This was coincided with a significant decrease in the culturable bacterial diversity of the granular biomass. PCR-DGGE analysis revealed a shift and decrease in number of bands during the establishment of PNP biodegradation. Scanning electron microscopy showed the dominance of rod-shaped bacteria in the PNP-utilizing microbial granules. Our results suggest that acetate-fed granular sludge could be quickly adapted for PNP biodegradation.

KW - Aerobic granular sludge

KW - Aerobic granules

KW - Biodegradation

KW - p-nitrophenol

UR - https://www.scopus.com/pages/publications/84858702727

U2 - 10.1007/s12010-011-9509-3

DO - 10.1007/s12010-011-9509-3

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AN - SCOPUS:84858702727

SN - 0273-2289

VL - 166

SP - 1225

EP - 1235

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 5

ER -

Rapid establishment of p-nitrophenol biodegradation in acetate-fed aerobic granular sludge

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Keywords

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