Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood

Ting Zhang; Ran Tao; Chunmei Yue; Naihe Jing

doi:10.1016/j.xpro.2021.100346

Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood

Ting Zhang
, Ran Tao
, Chunmei Yue^*
, Naihe Jing^*

^*Corresponding author for this work

Department of Biosciences and Bioinformatics

CAS - Shanghai Institute of Biochemistry and Cell Biology
Shanghai Jiao Tong University
National Clinical Research Center for Ophthalmic Diseases
Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)
CAS - Guangzhou Institute of Biomedicine and Health
Chinese Academy of Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).

Original language	English
Article number	100346
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100346
Publication status	Published - 19 Mar 2021

Keywords

Cell differentiation
Cell isolation
Neuroscience
Stem cells

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10.1016/j.xpro.2021.100346

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title = "Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood",

abstract = "Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).",

keywords = "Cell differentiation, Cell isolation, Neuroscience, Stem cells",

author = "Ting Zhang and Ran Tao and Chunmei Yue and Naihe Jing",

note = "Funding Information: This work was supported in part by the National Key Basic Research and Development Program of China ( 2019YFA0801402 , 2018YFA0800100 , 2018YFA0108000 , 2018YFA0107200 , 2017YFA0102700 ), {"}Strategic Priority Research Program{"} of the Chinese Academy of Sciences , grant no. ( XDA16020501 , XDA16020404 ), National Natural Science Foundation of China ( 81671224 , 31630043 ). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

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doi = "10.1016/j.xpro.2021.100346",

language = "English",

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TY - JOUR

T1 - Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood

AU - Zhang, Ting

AU - Tao, Ran

AU - Yue, Chunmei

AU - Jing, Naihe

N1 - Funding Information: This work was supported in part by the National Key Basic Research and Development Program of China ( 2019YFA0801402 , 2018YFA0800100 , 2018YFA0108000 , 2018YFA0107200 , 2017YFA0102700 ), "Strategic Priority Research Program" of the Chinese Academy of Sciences , grant no. ( XDA16020501 , XDA16020404 ), National Natural Science Foundation of China ( 81671224 , 31630043 ). Publisher Copyright: © 2021 The Author(s)

PY - 2021/3/19

Y1 - 2021/3/19

N2 - Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).

AB - Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2019).

KW - Cell differentiation

KW - Cell isolation

KW - Neuroscience

KW - Stem cells

UR - https://www.scopus.com/pages/publications/85122806299

U2 - 10.1016/j.xpro.2021.100346

DO - 10.1016/j.xpro.2021.100346

M3 - Article

C2 - 33665626

AN - SCOPUS:85122806299

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 100346

ER -

Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood

Abstract

Keywords

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