@inbook{5ba28b5ef5c44432a669699f844d41cb,
title = "NGS-based high-throughput screen to identify MicroRNAs regulating growth of B-cell lymphoma",
abstract = "MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.",
keywords = "B-cell lymphoma, Cell growth, High-throughput screen, MiRNA inhibition, MiRNA overexpression, MicroRNA",
author = "Joost Kluiver and Fubiao Niu and Ye Yuan and Klaas Kok and \{van den Berg\}, Anke and Agnieszka Dzikiewicz-Krawczyk",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9151-8\_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "269--282",
booktitle = "Methods in Molecular Biology",
}