Laser-Induced Olfactory Bulbectomy in Adult Zebrafish as a Novel Putative Model for Affective Syndrome: A Research Tribute to Brian Leonard

Inducing multiple neurobehavioural and neurochemical deficits, olfactory bulbectomy (OBX) has been developed as a rodent model of depression with potential for antidepressant drug screening. However, the generality of this model in other vertebrate taxa remains poorly understood. A small freshwater teleost fish, the zebrafish (Danio rerio), is rapidly becoming a common model species in neuroscience research. Capitalizing on a recently developed model of noninvasive targeted laser ablation of zebrafish brain, here we report an OBX model in adult fish. An easy-to-perform noninvasive method of inducing affective syndrome-like behavioural deficits in fish, it extends the generality of OBX to other taxa beyond mammals, also offering several practical advantages and novel lines of research in experimental modelling of CNS disorders. The work is a scientific tribute to the legacy of Brian Leonard (1936–2023), a great friend and a brilliant scientist who introduced OBX as a rodent model for affective pathobiology and whose advice and encouragement have inspired the present study.