Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants

Tatsuhiko Kadowaki; Shaoping Chen; Midori Hitomi; Erica Jacobs; Chino Kumagai; Shuang Liang; Roger Schneiter; David Singleton; Joanna Wisniewska; Alan M. Tartakoff

Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants

Tatsuhiko Kadowaki
, Shaoping Chen
, Midori Hitomi
, Erica Jacobs
, Chino Kumagai
, Shuang Liang
, Roger Schneiter
, David Singleton
, Joanna Wisniewska
, Alan M. Tartakoff^*

^*Corresponding author for this work

Case Western Reserve University

Research output: Contribution to journal › Article › peer-review

147 Citations (Scopus)

Abstract

To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)⁺ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 coming mutants, these strains do not interrupt splicing of pre-mRNA at 37°C; however four strains accumulate oversized RNA polymerase II transcripts. Some show inhibition of rRNA processing and a further subset of these strains is also characterized by inhibition of tRNA maturation. Several strains accumulate nuclear.

Original language	English
Pages (from-to)	649-659
Number of pages	11
Journal	Journal of Cell Biology
Volume	126
Issue number	3
Publication status	Published - Aug 1994
Externally published	Yes

Cite this

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title = "Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants",

abstract = "To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 coming mutants, these strains do not interrupt splicing of pre-mRNA at 37°C; however four strains accumulate oversized RNA polymerase II transcripts. Some show inhibition of rRNA processing and a further subset of these strains is also characterized by inhibition of tRNA maturation. Several strains accumulate nuclear.",

author = "Tatsuhiko Kadowaki and Shaoping Chen and Midori Hitomi and Erica Jacobs and Chino Kumagai and Shuang Liang and Roger Schneiter and David Singleton and Joanna Wisniewska and Tartakoff, \{Alan M.\}",

year = "1994",

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language = "English",

volume = "126",

pages = "649--659",

journal = "Journal of Cell Biology",

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T1 - Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants

AU - Kadowaki, Tatsuhiko

AU - Chen, Shaoping

AU - Hitomi, Midori

AU - Jacobs, Erica

AU - Kumagai, Chino

AU - Liang, Shuang

AU - Schneiter, Roger

AU - Singleton, David

AU - Wisniewska, Joanna

AU - Tartakoff, Alan M.

PY - 1994/8

Y1 - 1994/8

N2 - To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 coming mutants, these strains do not interrupt splicing of pre-mRNA at 37°C; however four strains accumulate oversized RNA polymerase II transcripts. Some show inhibition of rRNA processing and a further subset of these strains is also characterized by inhibition of tRNA maturation. Several strains accumulate nuclear.

AB - To understand the mechanisms of mRNA transport in eukaryotes, we have isolated Saccharomyces cerevisiae temperature-sensitive (ts) mutants which accumulate poly(A)+ RNA in the nucleus at the restrictive temperature. A total of 21 recessive mutants were isolated and classified into 16 coming mutants, these strains do not interrupt splicing of pre-mRNA at 37°C; however four strains accumulate oversized RNA polymerase II transcripts. Some show inhibition of rRNA processing and a further subset of these strains is also characterized by inhibition of tRNA maturation. Several strains accumulate nuclear.

UR - https://www.scopus.com/pages/publications/0027977982

M3 - Article

C2 - 8045930

AN - SCOPUS:0027977982

SN - 0021-9525

VL - 126

SP - 649

EP - 659

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 3

ER -

Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants

Abstract

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