Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals

Raju Sekar; Longin T. Kaczmarsky; Laurie L. Richardson

doi:10.1128/AEM.01500-08

Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals

Raju Sekar^*
, Longin T. Kaczmarsky
, Laurie L. Richardson

^*Corresponding author for this work

Florida International University
University of Sheffield

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

Original language	English
Pages (from-to)	2581-2584
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	8
DOIs	https://doi.org/10.1128/AEM.01500-08
Publication status	Published - Apr 2009
Externally published	Yes

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title = "Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals",

abstract = "Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.",

author = "Raju Sekar and Kaczmarsky, \{Longin T.\} and Richardson, \{Laurie L.\}",

year = "2009",

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language = "English",

volume = "75",

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journal = "Applied and Environmental Microbiology",

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T1 - Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals

AU - Sekar, Raju

AU - Kaczmarsky, Longin T.

AU - Richardson, Laurie L.

PY - 2009/4

Y1 - 2009/4

N2 - Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

AB - Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

UR - https://www.scopus.com/pages/publications/64749085263

U2 - 10.1128/AEM.01500-08

DO - 10.1128/AEM.01500-08

M3 - Article

C2 - 19251896

AN - SCOPUS:64749085263

SN - 0099-2240

VL - 75

SP - 2581

EP - 2584

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 8

ER -

Effect of freezing on PCR amplification of 16S rRNA genes from microbes associated with black band disease of corals

Abstract

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