Effect of cell membrane chlorine currents on energy metabolism of murine activated lymphocytes and bone marrow cells

A. M. Sapozhnikov; D. V. Baev; G. A. Gusarova; E. D. Ponomarev; I. M. Molotkovskaya

Effect of cell membrane chlorine currents on energy metabolism of murine activated lymphocytes and bone marrow cells

A. M. Sapozhnikov^*
, D. V. Baev
, G. A. Gusarova
, E. D. Ponomarev
, I. M. Molotkovskaya

^*Corresponding author for this work

Inst. Bioorganicheskoj Khimii
Rossiyskaya Akademiya Nauk

Research output: Contribution to journal › Article › peer-review

Abstract

Chlorine channels have been found in immune cells but their functions were unclear. We have used MTT-assay to investigate the effect of CI channel blockers and Cl removal on energy metabolism (EM) of ConA-activated lymphocytes, EL-4 cells and bone marrow cells. The Cl channel blockers SITS and DIDS inhibited EM of ConA-blasts and EL-4 cells in a dose-dependent manner. Bone marrow cells were more resistant to inhibitory action of SITS and DIDS. Moreover, low and middle doses of SIRS resulted in some activation of EM of these cells. Besides, Cl channel blockers increased the level of intracellular Ca²⁺ in bone marrow cells. Our results suggest that the function of Cl channels in bone marrow precursors differs from that in more mature immune cells.

Original language	English
Pages (from-to)	37-41
Number of pages	5
Journal	Immunologiya
Issue number	4
Publication status	Published - 1999
Externally published	Yes

Cite this

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title = "Effect of cell membrane chlorine currents on energy metabolism of murine activated lymphocytes and bone marrow cells",

abstract = "Chlorine channels have been found in immune cells but their functions were unclear. We have used MTT-assay to investigate the effect of CI channel blockers and Cl removal on energy metabolism (EM) of ConA-activated lymphocytes, EL-4 cells and bone marrow cells. The Cl channel blockers SITS and DIDS inhibited EM of ConA-blasts and EL-4 cells in a dose-dependent manner. Bone marrow cells were more resistant to inhibitory action of SITS and DIDS. Moreover, low and middle doses of SIRS resulted in some activation of EM of these cells. Besides, Cl channel blockers increased the level of intracellular Ca2+ in bone marrow cells. Our results suggest that the function of Cl channels in bone marrow precursors differs from that in more mature immune cells.",

author = "Sapozhnikov, \{A. M.\} and Baev, \{D. V.\} and Gusarova, \{G. A.\} and Ponomarev, \{E. D.\} and Molotkovskaya, \{I. M.\}",

year = "1999",

language = "English",

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journal = "Immunologiya",

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TY - JOUR

T1 - Effect of cell membrane chlorine currents on energy metabolism of murine activated lymphocytes and bone marrow cells

AU - Sapozhnikov, A. M.

AU - Baev, D. V.

AU - Gusarova, G. A.

AU - Ponomarev, E. D.

AU - Molotkovskaya, I. M.

PY - 1999

Y1 - 1999

N2 - Chlorine channels have been found in immune cells but their functions were unclear. We have used MTT-assay to investigate the effect of CI channel blockers and Cl removal on energy metabolism (EM) of ConA-activated lymphocytes, EL-4 cells and bone marrow cells. The Cl channel blockers SITS and DIDS inhibited EM of ConA-blasts and EL-4 cells in a dose-dependent manner. Bone marrow cells were more resistant to inhibitory action of SITS and DIDS. Moreover, low and middle doses of SIRS resulted in some activation of EM of these cells. Besides, Cl channel blockers increased the level of intracellular Ca2+ in bone marrow cells. Our results suggest that the function of Cl channels in bone marrow precursors differs from that in more mature immune cells.

AB - Chlorine channels have been found in immune cells but their functions were unclear. We have used MTT-assay to investigate the effect of CI channel blockers and Cl removal on energy metabolism (EM) of ConA-activated lymphocytes, EL-4 cells and bone marrow cells. The Cl channel blockers SITS and DIDS inhibited EM of ConA-blasts and EL-4 cells in a dose-dependent manner. Bone marrow cells were more resistant to inhibitory action of SITS and DIDS. Moreover, low and middle doses of SIRS resulted in some activation of EM of these cells. Besides, Cl channel blockers increased the level of intracellular Ca2+ in bone marrow cells. Our results suggest that the function of Cl channels in bone marrow precursors differs from that in more mature immune cells.

UR - https://www.scopus.com/pages/publications/0032875154

M3 - Article

AN - SCOPUS:0032875154

SN - 0206-4952

SP - 37

EP - 41

JO - Immunologiya

JF - Immunologiya

IS - 4

ER -

Effect of cell membrane chlorine currents on energy metabolism of murine activated lymphocytes and bone marrow cells

Abstract

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