Development of A Novel NanoBRET High-Throughput Drug Screening Assay for Human GnRH Receptor Using Sulfo-cyanine 5 Fluorophore

Li Shen; Xiaozhe Du; Yakai Yang; Ming Su; Rong Rong; Jia Meng; Lee Wei Lim; David Fernig; Zhiliang Lu

Development of A Novel NanoBRET High-Throughput Drug Screening Assay for Human GnRH Receptor Using Sulfo-cyanine 5 Fluorophore

Li Shen
, Xiaozhe Du
, Yakai Yang
, Ming Su
, Rong Rong
, Jia Meng
, Lee Wei Lim
, David Fernig
, Zhiliang Lu^*

^*Corresponding author for this work

University of Liverpool

Research output: Contribution to journal › Article › peer-review

Original language	English
Journal	Journal of Pharmaceutical Analysis
Publication status	Accepted/In press - 2025

Keywords

G protein-coupled receptors, high throughput drug screening; NanoBRET; fluorescent ligand; gonadotrophin-releasing hormone (GnRH); GnRH receptor

2 Finished
2 Active

Suzhou Municipal Key Lab of Cancer Biology and Chronic Disease
Lu, Z. (PI)
1/04/24 → …
Project: Governmental Research Project
Roles of Lac-Phe and G proteins inthe regulation of m6A mRNA methylation
Lu, Z. (PI)
1/03/24 → 31/12/28
Project: Internal Research Project
Examination of G protein coupling profile of human GnRH receptor and its P128K mutant
Lu, Z. (PI)
1/01/23 → 31/12/25
Project: Internal Research Project

Cite this

@article{eb5f79f37cd147418bd1277ab96e2a07,

title = "Development of A Novel NanoBRET High-Throughput Drug Screening Assay for Human GnRH Receptor Using Sulfo-cyanine 5 Fluorophore",

keywords = "G protein-coupled receptors, high throughput drug screening; NanoBRET; fluorescent ligand; gonadotrophin-releasing hormone (GnRH); GnRH receptor",

author = "Li Shen and Xiaozhe Du and Yakai Yang and Ming Su and Rong Rong and Jia Meng and Lim, \{Lee Wei\} and David Fernig and Zhiliang Lu",

year = "2025",

language = "English",

journal = "Journal of Pharmaceutical Analysis",

issn = "2095-1779",

publisher = "Elsevier",

}

TY - JOUR

T1 - Development of A Novel NanoBRET High-Throughput Drug Screening Assay for Human GnRH Receptor Using Sulfo-cyanine 5 Fluorophore

AU - Shen, Li

AU - Du, Xiaozhe

AU - Yang, Yakai

AU - Su, Ming

AU - Rong, Rong

AU - Meng, Jia

AU - Lim, Lee Wei

AU - Fernig, David

AU - Lu, Zhiliang

PY - 2025

Y1 - 2025

KW - G protein-coupled receptors, high throughput drug screening; NanoBRET; fluorescent ligand; gonadotrophin-releasing hormone (GnRH); GnRH receptor

M3 - Article

SN - 2095-1779

JO - Journal of Pharmaceutical Analysis

JF - Journal of Pharmaceutical Analysis

ER -

Development of A Novel NanoBRET High-Throughput Drug Screening Assay for Human GnRH Receptor Using Sulfo-cyanine 5 Fluorophore

Keywords

Projects

Suzhou Municipal Key Lab of Cancer Biology and Chronic Disease

Roles of Lac-Phe and G proteins inthe regulation of m6A mRNA methylation

Examination of G protein coupling profile of human GnRH receptor and its P128K mutant

Cite this